- Record: found
- Abstract: not found
- Article: not found
An enzyme cocktail for efficient protoplast formation in Aspergillus niger
March 2009
March 2009
There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation
of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to
protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma
harzianum, chitinase from Streptomyces griseus and beta-glucuronidase from Helix pomatia.