The belief that the anabolic response to feeding during postexercise recovery is transient and has an upper limit and that excess amino acids are being oxidized lacks scientific proof. Using a comprehensive quadruple isotope tracer feeding-infusion approach, we show that the ingestion of 100 g protein results in a greater and more prolonged (>12 h) anabolic response when compared to the ingestion of 25 g protein. We demonstrate a dose-response increase in dietary-protein-derived plasma amino acid availability and subsequent incorporation into muscle protein. Ingestion of a large bolus of protein further increases whole-body protein net balance, mixed-muscle, myofibrillar, muscle connective, and plasma protein synthesis rates. Protein ingestion has a negligible impact on whole-body protein breakdown rates or amino acid oxidation rates. These findings demonstrate that the magnitude and duration of the anabolic response to protein ingestion is not restricted and has previously been underestimated in vivo in humans.
Postprandial protein anabolism remains elevated during prolonged hyperaminoacidemia
Protein ingestion has a negligible impact on whole-body amino acid oxidation
Muscle protein autophagy is not modulated by protein ingestion
Exogenous amino acids are the main precursors in postprandial protein accretion
Trommelen et al. investigate the anabolic response to the ingestion of various amounts of protein following exercise and demonstrate a dose-response increase in the magnitude and duration of protein absorption, muscle protein synthesis rates, and whole-body net protein balance. The anabolic response to protein ingestion has no upper limit.