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      Multipronged dental analyses reveal dietary differences in last foragers and first farmers at Grotta Continenza, central Italy (15,500–7000 BP)

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          Abstract

          This paper provides results from a suite of analyses made on human dental material from the Late Palaeolithic to Neolithic strata of the cave site of Grotta Continenza situated in the Fucino Basin of the Abruzzo region of central Italy. The available human remains from this site provide a unique possibility to study ways in which forager versus farmer lifeways affected human odonto-skeletal remains. The main aim of our study is to understand palaeodietary patterns and their changes over time as reflected in teeth. These analyses involve a review of metrics and oral pathologies, micro-fossils preserved in the mineralized dental plaque, macrowear, and buccal microwear. Our results suggest that these complementary approaches support the assumption about a critical change in dental conditions and status with the introduction of Neolithic foodstuff and habits. However, we warn that different methodologies applied here provide data at different scales of resolution for detecting such changes and a multipronged approach to the study of dental collections is needed for a more comprehensive and nuanced understanding of diachronic changes.

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          ROBPCA: A New Approach to Robust Principal Component Analysis

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            Patterns of molar wear in hunger-gatherers and agriculturalists.

            Tooth wear records valuable information on diet and methods of food preparation in prehistoric populations or extinct species. In this study, samples of modern and prehistoric hunger-gatherers and agriculturalists are used to test the hypothesis that there are systematic differences in patterns of tooth wear related to major differences in subsistence and food preparation. Flatness of molar wear is compared for five groups in hunger-gatherers (N = 298) and five groups of early agriculturalists (N = 365). Hunger-gatherers are predicted to develop flatter molar wear due to the mastication of tough and fibrous foods, whereas agriculturalists should develop oblique molar wear due to an increase in the proportion of ground and prepared food in the diet. A method is presented for the quantitative measurement and analysis of flatness of molar wear. Comparisons of wear plane angle are made between teeth matched for the same stage of occlusal surface wear, thus standardizing all groups to the same rate of wear. Agriculturalists develop highly angled occlusal wear planes on the entire molar dentition. Their wear plane angles tend to exceed hunger-gatherers by about 10 degrees in advanced wear. Wear plane angles are similar within subsistence divisions despite regional differences in particular foods. This approach can be used to provide supporting evidence of change in human subsistence and to test dietary hypotheses in hominoid evolution.
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              Processing of wild cereal grains in the Upper Palaeolithic revealed by starch grain analysis.

              Barley (Hordeum vulgare L.) and wheat (Triticum monococcum L. and Triticum turgidum L.) were among the principal 'founder crops' of southwest Asian agriculture. Two issues that were central to the cultural transition from foraging to food production are poorly understood. They are the dates at which human groups began to routinely exploit wild varieties of wheat and barley, and when foragers first utilized technologies to pound and grind the hard, fibrous seeds of these and other plants to turn them into easily digestible foodstuffs. Here we report the earliest direct evidence for human processing of grass seeds, including barley and possibly wheat, in the form of starch grains recovered from a ground stone artefact from the Upper Palaeolithic site of Ohalo II in Israel. Associated evidence for an oven-like hearth was also found at this site, suggesting that dough made from grain flour was baked. Our data indicate that routine processing of a selected group of wild cereals, combined with effective methods of cooking ground seeds, were practiced at least 12,000 years before their domestication in southwest Asia.
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                Author and article information

                Contributors
                emanuela.cristiani@uniroma1.it
                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group UK (London )
                2045-2322
                19 February 2021
                19 February 2021
                2021
                : 11
                : 4261
                Affiliations
                [1 ]GRID grid.7841.a, Department of Maxillo-Facial Sciences, DANTE - Diet and Ancient Technology Laboratory, , Sapienza University of Rome, ; Rome, Italy
                [2 ]GRID grid.9759.2, ISNI 0000 0001 2232 2818, Skeletal Biology Research Centre, School of Anthropology and Conservation, , University of Kent, ; Canterbury, UK
                [3 ]GRID grid.7841.a, Department of Maxillo-Facial Sciences, , Sapienza University of Rome, ; Rome, Italy
                [4 ]Bioarchaeology Service, Museum of Civilizations, Rome, Italy
                [5 ]GRID grid.5395.a, ISNI 0000 0004 1757 3729, Department of Archaeological Science, , University of Pisa, ; Pisa, Italy
                [6 ]GRID grid.7841.a, Department of Environmental Biology, , Sapienza University of Rome, ; Rome, Italy
                [7 ]GRID grid.5608.b, ISNI 0000 0004 1757 3470, Department of Cultural Heritage, , University of Padua, ; Padua, Italy
                [8 ]GRID grid.21729.3f, ISNI 0000000419368729, The Italian Academy for Advanced Studies in America, , Columbia University, ; New York, USA
                [9 ]GRID grid.38142.3c, ISNI 000000041936754X, Department of Genetics, Harvard Medical School, , Harvard University, ; Cambridge, USA
                [10 ]GRID grid.10420.37, ISNI 0000 0001 2286 1424, Department of Evolutionary Anthropology, , University of Vienna, ; Vienna, Austria
                Author information
                http://orcid.org/0000-0002-6319-0425
                http://orcid.org/0000-0003-4295-1026
                http://orcid.org/0000-0002-9503-1234
                http://orcid.org/0000-0001-5656-9157
                http://orcid.org/0000-0001-8870-1589
                http://orcid.org/0000-0002-8039-7022
                http://orcid.org/0000-0001-8545-9889
                http://orcid.org/0000-0003-0166-627X
                http://orcid.org/0000-0002-2748-9171
                Article
                82401
                10.1038/s41598-021-82401-2
                7895915
                33608594
                3d76a791-4d71-40b3-9cc6-aea5c006654e
                © The Author(s) 2021

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 15 September 2020
                : 22 December 2020
                Funding
                Funded by: FundRef http://dx.doi.org/10.13039/100010661, Horizon 2020 Framework Programme;
                Award ID: G.A. 639286
                Funded by: Sapienza University of Rome - Bandi per la Ricerca di Ateneo
                Award ID: no. 680595 - Prot. RM11715C64171FC6
                Funded by: FundRef http://dx.doi.org/10.13039/501100008483, NOMIS Stiftung;
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                © The Author(s) 2021

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                archaeology
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                archaeology

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