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      FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut Bacterium Akkermansia muciniphila.

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          Abstract

          Based on their unique properties, oligonucleotide aptamers have been named a gift of biological chemistry to life science. We report the development of DNA aptamers as the first high-affinity binding molecules available for fast and rapid labeling of the human gut bacterium Akkermansia muciniphila with a certain impact on Alzheimer´s disease. Fast and reliable analyses of the composition of microbiomes is an emerging field in microbiology. We describe the molecular evolution and biochemical characterization of a specific aptamer library by a FluCell-SELEX and the characterization of specific molecules from the library by bioinformatics. The aptamer AKK13.1 exerted universal applicability in different analysis techniques in modern microbiology, including fluorimetry, confocal laser scanning microscopy and flow cytometry. It was also functional as a specific binding entity hybridized to anchor primers chemically coupled via acrydite-modification to the surface of a polyacrylamide-hydrogel, which can be prototypically used for the construction of affinity surfaces in sensor chips. Together, the performance and methodological flexibility of the aptamers presented here may open new routes not only to develop novel Akkermansia-specific assays for clinical microbiology and the analyses of human stool samples but may also be an excellent starting point for the construction of novel electronic biosensors.

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          Author and article information

          Journal
          Int J Mol Sci
          International journal of molecular sciences
          MDPI AG
          1422-0067
          1422-0067
          Sep 27 2021
          : 22
          : 19
          Affiliations
          [1 ] Institute for Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
          [2 ] Max Planck Institute for Polymer Research Mainz, Ackermannweg 10, 55128 Mainz, Germany.
          [3 ] Institute for Medical Microbiology and Hygiene, University Hospital Ulm, 89081 Ulm, Germany.
          [4 ] Department of General and Visceral Surgery, Surgery Center, Ulm University, Albert-Einstein-Allee 23, 89081 Ulm, Germany.
          Article
          ijms221910425
          10.3390/ijms221910425
          8509069
          34638764
          3d15be15-df62-41c2-8cf2-9c51a819f6e5
          History

          FluCell-SELEX,aptamers,biosensors
          FluCell-SELEX, aptamers, biosensors

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