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      Evaluation of MALDI Biotyper Mycobacteria Library v3.0 for Identification of Nontuberculous Mycobacteria

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          Abstract

          Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has demonstrated its ability to promptly identify nontuberculous mycobacteria using the Mycobacteria Library v2.0. However, some species are particularly difficult to identify reliably using this database, providing a low log(score). In this study, the identification power of an updated Mycobacteria Library (v3.0) has been evaluated. Overall, 109 NTM isolates were analyzed with both databases. The v3.0 database allowed a high-level confidence in the identification [log(score) value, ≥1.8] of 91.7% of the isolates versus 83.5% with the v2.0 version ( P < 0.01).

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          Author and article information

          Contributors
          Role: Editor
          Journal
          J Clin Microbiol
          J. Clin. Microbiol
          jcm
          jcm
          JCM
          Journal of Clinical Microbiology
          American Society for Microbiology (1752 N St., N.W., Washington, DC )
          0095-1137
          1098-660X
          3 February 2016
          25 March 2016
          April 2016
          : 54
          : 4
          : 1144-1147
          Affiliations
          [a ]Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain
          [b ]CIBER de Enfermedades Respiratorias (CIBERES CB06/06/0058), Madrid, Spain
          [c ]Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain
          [d ]Bruker Daltonik GmbH, Bremen, Germany
          [e ]Medicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain
          Author notes
          Address correspondence to Belén Rodríguez-Sánchez, mbelen.rodriguez@ 123456iisgm.com .

          Citation Rodríguez-Sánchez B, Ruiz-Serrano MJ, Ruiz A, Timke M, Kostrzewa M, Bouza E. 2016. Evaluation of MALDI Biotyper Mycobacteria Library v3.0 for identification of nontuberculous mycobacteria. J Clin Microbiol 54:1144–1147. doi: 10.1128/JCM.02760-15.

          Article
          PMC4809906 PMC4809906 4809906 02760-15
          10.1128/JCM.02760-15
          4809906
          26842704
          3c2d669c-cf5e-46a2-9788-35e8070e7cf3
          Copyright © 2016, American Society for Microbiology. All Rights Reserved.
          History
          : 13 October 2015
          : 7 November 2015
          : 23 January 2016
          Page count
          Figures: 0, Tables: 3, Equations: 0, References: 7, Pages: 4, Words: 3439
          Funding
          Funded by: Miguel Servet Program (ISCIII_MICINN)
          Award ID: CP14/00220
          Award Recipient : Belen Rodriguez-Sanchez
          The Miguel Servet Program is a governmental program to promote emerging scientists. This study was supported by the Miguel Servet Program CP14/00220, part of the Plan Estatal I+D+I 2013-2016, and cofinanced by ISCII-Subdirección General de Evaluación y Fomento de la Investigación and the European Regional Development Fund.
          Categories
          Mycobacteriology and Aerobic Actinomycetes

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