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      Estudio del estrés oxidativo en 2 cepas de Euglena gracilis

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          Abstract

          Se realizaron estudios de estrés oxidativo en 2 cepas de Euglena gracilis (MAT y UTEX). Se sometieron los cultivos a diferentes concentraciones de cromo hexavalente. Se determinó la actividad superóxido dismutasa, la cantidad de lípidos totales, la concentración de clorofila a y se realizaron las respectivas curvas de crecimiento. Los resultados obtenidos demuestran que el cromo, además de afectar la producción de lípidos, clorofila y crecimiento celular, aumenta la actividad superóxido dismutasa y se observan notables diferencias entre ambas cepas.

          Translated abstract

          Studies of oxidative stress were conducted in 2 strains of Euglena gracilis (MAT and UTEX). The cultures were subjected to different concentrations of hexavalent chromium. The superoxide dismutase activity, the amount of total lipids and the concentration of clorophyll were determined and the respective growth curves were obtained. The results obtained show that chromium besides affecting the production of lipids, clorophyll and cellular growth, increases the superoxide dismutase activity. Significant differences are observed between both strains.

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          The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase.

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            The role of hydroxyl radical as a messenger in Cr(VI)-induced p53 activation.

            The present study investigates whether reactive oxygen species (ROS) are involved in p53 activation, and if they are, which species is responsible for the activation. Our hypothesis is that hydroxyl radical (.OH) functions as a messenger for the activation of this tumor suppressor protein. Human lung epithelial cells (A549) were used to test this hypothesis. Cr(VI) was employed as the source of ROS due to its ability to generate a whole spectrum of ROS inside the cell. Cr(VI) is able to activate p53 by increasing the protein levels and enhancing both the DNA binding activity and transactivation ability of the protein. Increased cellular levels of superoxide radicals (O(2)(-).), hydrogen peroxide (H(2)O(2)), and.OH radicals were detected on the addition of Cr(VI) to the cells. Superoxide dismutase, by enhancing the production of H(2)O(2) from O(2)(-). radicals, increased p53 activity. Catalase, an H(2)O(2) scavenger, eliminated.OH radical generation and inhibited p53 activation. Sodium formate and aspirin,.OH radical scavengers, also suppressed p53 activation. Deferoxamine, a metal chelator, inhibited p53 activation by chelating Cr(V) to make it incapable of generating radicals from H(2)O(2). NADPH, which accelerated the one-electron reduction of Cr(VI) to Cr(V) and increased.OH radical generation, dramatically enhanced p53 activation. Thus.OH radical generated from Cr(VI) reduction in A549 cells is responsible for Cr(VI)-induced p53 activation.
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              Cytotoxic effects of hexavalent chromium inEuglena gracilis. II. Physiological and ultrastructural studies

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                Author and article information

                Journal
                ibi
                Revista Cubana de Investigaciones Biomédicas
                Rev Cubana Invest Bioméd
                ECIMED (Ciudad de la Habana, , Cuba )
                0864-0300
                1561-3011
                June 2003
                : 22
                : 2
                : 105-108
                Affiliations
                [01] Buenos Aires orgnameDepartamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires Argentina mcrios@ 123456qb.fcen.uba.ar
                Article
                S0864-03002003000200005 S0864-0300(03)02200205
                3b95fa7d-9b30-49aa-8087-e24df8180dc3

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 11 October 2001
                : 15 December 2002
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 8, Pages: 4
                Product

                SciELO Cuba

                Categories
                TRABAJOS ORIGINALES

                CLOROPHYLL,CHROMIUM,EUGLENA GRACILIS,OXIDATIVE STRESS,CLOROFILA,CROMO,ESTRÉS OXIDATIVO

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