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      Comportamiento metabolico en el periparto de vacas Hartón del Valle, bajo condiciones de trópico bajo Translated title: Metabolic behaviour in the peripartum period of dairy cows Hartón del Valle creole breed, under tropical conditions

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          Abstract

          El periodo de periparto o periodo de transición presenta variaciones fisiológicas significativas que inciden en posteriores sucesos productivos (pico de lactancia, reactivación ovárica). El ganado Hartón posee una excelente eficiencia reproductiva (estrecho intervalo entre partos), factor que podría originarse en procesos metabólicos relacionados con un mejor ajuste homeostático. El principal objetivo del presente trabajo fue evaluar la homeostasis en una raza bovina criolla durante el periodo postparto. Para el estudio se emplearon 10 vacas multíparas, muestreadas antes del parto (días 30 y 15 preparto) y diariamente en los tres primeros días del parto; posteriormente a partir del día 5, con intervalos de cinco días y hasta el día 60 postparto. En total para cada animal se analizaron 14 muestras, cada una de ellas correspondió a un periodo analítico. Mediante venipunción coccígea en tubos al vacío con y sin anticoagulante se colectó la muestra de sangre completa. Por centrifugación se obtuvo plasma y suero, los cuales fueron almacenados a -20°C, hasta su análisis. Las determinaciones de hormonas se realizaron mediante radioinmunoanálisis (RIA) de fase sólida. A través de pruebas enzimáticas colorimétricas en equipos automatizados, se determinaron los valores de los metabolitos para cada uno de los periodos definidos. Los valores medios fueron: BHB (β-Hidroxibutirato) 0.39 mmol/L, NEFA (ácidos Grasos No Esterificados) 0.76 mmol/L, triglicéridos 0.42 mmol/L, colesterol 2.43 mmol/L, insulina 4.77 mµl/ml, triyodotironina (T3) 2.69 nmol/L, tetrayodotironina (T4) 57.37 nmol/L, progesterona 5.54 nmol/L, cortisol 32.42 nmol/L. Los valores obtenidos para los diferentes metabolitos se encuentran dentro de los rangos reportados por la literatura para bovinos en condiciones tropicales.

          Translated abstract

          The peripartum period or transition period, presents significant physiological changes that affect production in subsequent production events (peak lactation, ovarian resumption). Creole bovine cattle "Hartón del Valle" has an excellent reproductive performance (short iterpartum period); this factor could have been originated from metabolic processes related to an improved homeostatic adjustment. The main aim of this study was to assess the homeostasis in a native cattle breed in the postpartum period. For this study, 10 multiparous cows were used and sampled before birth (days 30 and 15 prepartum) and daily in the first three days of birth, and afterwards from day 5, at intervals of five days and up to-day 60 postpartum. In total, for each animal 14 samples were analyzed which correspond to one analysis period. Using the coccygeal venipuncture technic, in vacuum tubes with and without anticoagulant the complete sample was collected. By centrifugation, plasma and serum were obtained and stored at -20°C until the metabolic analysis was done. Using a radioimmunoassay (RIA) of solid phase, hormone analysis were done. Through enzymatic colorimeter test in automated equipment the values of the metabolites for each of the defined period were determined. The mean values were: BHB (β-Hidroxibutirates) 0.39 mmol/L, NEFA (Non Esterified Fatid Acids) 0.76 mmol/L, triglycerides 0.42 mmol/L, cholesterol 2.43 mmol/L, triiodothyronine (T3) 2.69 nmol/L, tetraiodothyronine (T4) 57.37 nmol/L, insulin 4.77 mµl/ml, progesterone 5.54 nmol/L, cortisol 32.42 nmol/L. The values obtained for the different metabolites were within the biological ranges reported in the literature for cattle of tropical ecosystems.

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          Most cited references28

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          Biology of Dairy Cows During the Transition Period: the Final Frontier?

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            Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro.

            In this study concentration and composition of non-esterified fatty acids (NEFA) in follicular fluid (FF) of high-yielding dairy cows were determined during the period of negative energy balance (NEB) early post partum. NEFA were then added during in vitro maturation at concentrations measured previously in FF to evaluate their effect on the oocyte's developmental competence. At 16 and 44 days post partum, FF of the dominant follicle and blood were collected from nine high-yielding dairy cows. Samples were analysed for NEFA concentration and composition. NEFA concentrations in FF (0.2-0.6 mmol/l) during NEB remained +/- 40% lower compared with serum (0.4-1.2 mmol/l). The NEFA composition differed significantly between serum and FF with oleic acid (OA), palmitic acid (PA) and stearic acid (SA) being the predominant fatty acids in FF. Based on these results, 5115 oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of 0.200 mmol/l OA, 0.133 mmol/l PA or 0.067 mmol/l SA dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in SOF medium. Addition of PA or SA during oocyte maturation had negative effects on maturation, fertilization and cleavage rate and blastocyst yield. More (late) apoptotic cumulus cells were observed in cumulus-oocyte complexes matured in the presence of SA or PA. Ethanol or OA had no effect. These in vitro results suggest that NEB may hamper fertility of high-yielding dairy cows through increased NEFA concentrations in FF affecting oocyte quality.
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              Ketone bodies in milk and blood of dairy cows: relationship between concentrations and utilization for detection of subclinical ketosis.

              The objective of this study was to investigate the relationship between the concentrations of the different ketone bodies in milk and blood and to evaluate these concentrations for the detection of subclinical ketosis. A total of 60 multiparous cows were used. Concentrations of acetone, acetoacetate, and beta-hydroxybutyrate were analyzed quantitatively in blood and milk, and the Ketolac strip test was used for semiquantitative determination of beta-hydroxybutyrate in milk. Cows were defined subclinically ketotic when their concentration of blood beta-hydroxybutyrate was over 1200 micromol/L. High correlation coefficients were observed between blood acetone and blood acetoacetate, and between blood and milk acetone. On the contrary, concentrations of milk and blood beta-hydroxybutyrate were poorly correlated with the other concentrations of ketone bodies. The Ketolac strip test overestimated the concentrations of beta-hydroxybutyrate in milk. For the detection of subclinical ketosis, the best sensitivity-specificity combination was obtained with the determination of acetoacetate in blood or milk, with threshold concentrations of 125 and 50 micromol/L, respectively. Determination of beta-hydroxybutyrate in the milk via an enzymatic analysis or via the Ketolac strip test provided valuable results, with threshold concentrations of 70 and 100 micromol/L, respectively. The simplicity of use of the Ketolac strip test makes it a valuable way to investigate subclinical ketosis.
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                Author and article information

                Journal
                acag
                Acta Agronómica
                Acta Agron.
                Universidad Nacional de Colombia (Palmira, Valle, Colombia )
                0120-2812
                January 2011
                : 60
                : 1
                : 12-26
                Affiliations
                [01] Palmira Valle del Cauca orgnameUniversidad Nacional de Colombia orgdiv1Facultad de Ciencias Agropecuarias orgdiv2Departamento de Ciencia Animal Colombia rcamposgunal.edu.co, eahernandezpalmira.unal.edu.co, lgiraldopa@ 123456palmira.unal.edu.co
                Article
                S0120-28122011000100002 S0120-2812(11)06000100002
                39578ffc-57de-45a8-95b8-a39ff3969749

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 18 March 2011
                : 11 June 2010
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 31, Pages: 15
                Product

                SciELO Colombia

                Self URI: Texto completo solamente en formato PDF (ES)
                Categories
                Articulos Originales

                transition period,Adaptación,bovinos criollos,perfil metabólico,periodo de transición,periparto,Adaptation,creole cattle,metabolic profile

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