Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology

Abstract

The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes, circuits, and even entire genomes by gene synthesis methods has become an enabling technology for modern molecular biology and enables the design, build, test, learn, and repeat cycle underpinning innovations in synthetic biology. In this perspective, we briefly review the techniques and technologies that enable the synthesis of DNA oligonucleotides and their assembly into larger DNA constructs with a focus on recent advancements that have sought to reduce synthesis cost and increase sequence fidelity. The development of lower-cost methods to produce high-quality synthetic DNA will allow for the exploration of larger biological hypotheses by lowering the cost of use and help to close the DNA read–write cost gap.

Abstract

DNA oligonucleotides are typically synthesized using phosphoramidite chemistry methods and then assembled into larger constructs by a variety of methods. Recent advances have sought to reduce cost and increase sequence fidelity.