For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
25
views
85
references
 Top references
cited by
28
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      408
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Mesenchymal stem cell-derived extracellular vesicles prevent the development of osteoarthritis via the circHIPK3/miR-124-3p/MYH9 axis

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Extracellular vesicles (EVs) secreted by mesenchymal stem cells (MSCs) may play a vital role in a variety of biological processes, including cartilage regeneration. However, few studies reported their potential in the development of osteoarthritis (OA) previously. In this study, we explored the biological roles and underlying mechanism of MSCs-EVs in OA.

          Results

          Co-culture experiments revealed that MSCs-EVs could promote the expression of collagen type II alpha 1 chain (COL2A1), SRY-box transcription factor 9 (SOX9) and Aggrecan while negatively regulate the expression of chondrocyte hypertrophy markers matrix metallopeptidase 13 (MMP-13) and RUNX family transcription factor 2 (Runx2) in mouse chondrocytes in the OA model. Besides, the results of cell experiments indicated that MSCs-EVs could notably weaken the suppression of chondrocyte proliferation, migration and the promotion of chondrocyte apoptosis via interleukin1β (IL-1β) induction. In addition, MSCs-circHIPK3-EVs (EVs derived from MSCs overexpressing circHIPK3) considerably improved IL-1β-induced chondrocyte injury. Mechanistically, we elucidated that circHIPK3 could directly bind to miR-124-3p and subsequently elevate the expression of the target gene MYH9.

          Conclusion

          The findings in our study demonstrated that EVs-circHIPK3 participated in MSCs-EVs-mediated chondrocyte proliferation and migration induction and in chondrocyte apoptosis inhibition via the miR-124-3p/MYH9 axis. This offers a promising novel cell-free therapy for treating OA.

          Graphic abstract

          Related collections

          Most cited references85

          • Record: found
          • Abstract: found
          • Article: not found

          The biogenesis, biology and characterization of circular RNAs

          Lasse S. Kristensen, Maria Andersen, Lotte V. W. Stagsted … (2019)
          Circular RNAs (circRNAs) are covalently closed, endogenous biomolecules in eukaryotes with tissue-specific and cell-specific expression patterns, whose biogenesis is regulated by specific cis-acting elements and trans-acting factors. Some circRNAs are abundant and evolutionarily conserved, and many circRNAs exert important biological functions by acting as microRNA or protein inhibitors ('sponges'), by regulating protein function or by being translated themselves. Furthermore, circRNAs have been implicated in diseases such as diabetes mellitus, neurological disorders, cardiovascular diseases and cancer. Although the circular nature of these transcripts makes their detection, quantification and functional characterization challenging, recent advances in high-throughput RNA sequencing and circRNA-specific computational tools have driven the development of state-of-the-art approaches for their identification, and novel approaches to functional characterization are emerging.
          Article 0 comments Cited 1708 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            The expanding regulatory mechanisms and cellular functions of circular RNAs

            Ling-Ling Chen (2020)
            Article 0 comments Cited 570 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: found
              Is Open Access

              Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs

              Qiupeng Zheng, Chunyang Bao, Weijie Guo … (2016)
              Circular RNAs (circRNAs) represent a class of widespread and diverse endogenous RNAs that may regulate gene expression in eukaryotes. However, the regulation and function of human circRNAs remain largely unknown. Here we generate ribosomal-depleted RNA sequencing data from six normal tissues and seven cancers, and detect at least 27,000 circRNA candidates. Many of these circRNAs are differently expressed between the normal and cancerous tissues. We further characterize one abundant circRNA derived from Exon2 of the HIPK3 gene, termed circHIPK3. The silencing of circHIPK3 but not HIPK3 mRNA significantly inhibits human cell growth. Via a luciferase screening assay, circHIPK3 is observed to sponge to 9 miRNAs with 18 potential binding sites. Specifically, we show that circHIPK3 directly binds to miR-124 and inhibits miR-124 activity. Our results provide evidence that circular RNA produced from precursor mRNA may have a regulatory role in human cells.
              Article 0 comments Cited 553 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                Xiaohong Wang: wangxiaohong709@163.com , wangxiaohong@mail.tsinghua.edu.cn
                Journal
                Journal ID (nlm-ta): J Nanobiotechnology
                Journal ID (iso-abbrev): J Nanobiotechnology
                Title: Journal of Nanobiotechnology
                Publisher: BioMed Central (London )
                ISSN (Electronic): 1477-3155
                Publication date (Electronic): 30 June 2021
                Publication date PMC-release: 30 June 2021
                Publication date Collection: 2021
                Volume: 19
                Electronic Location Identifier: 194
                Affiliations
                [1 ]GRID grid.412449.e, ISNI 0000 0000 9678 1884, Department of Tissue Engineering, Center of 3D Printing & Organ Manufacturing, School of Fundamental Sciences, , China Medical University (CMU), ; No. 77 Puhe Road, Shenyang North New Area, Shenyang, 110122 China
                [2 ]GRID grid.459742.9, ISNI 0000 0004 1798 5889, Department of Bone and Soft Tissue Tumor Surgery, , Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, ; Shenyang, 110042 Liaoning Province China
                [3 ]GRID grid.412449.e, ISNI 0000 0000 9678 1884, Department of Prosthodontics, School and Hospital of Stomatology, , China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, ; Shenyang, 110002 China
                [4 ]GRID grid.12527.33, ISNI 0000 0001 0662 3178, Center of Organ Manufacturing, Department of Mechanical Engineering, , Tsinghua University, ; Beijing, 100084 China
                Article
                Publisher ID: 940
                DOI: 10.1186/s12951-021-00940-2
                PMC ID: 8244143
                PubMed ID: 34193158
                SO-VID: 3533bd67-4211-42cc-823d-ff5132716d25
                Copyright © © The Author(s) 2021
                License:

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

                History
                Date received : 15 May 2021
                Date accepted : 20 June 2021
                Categories
                Subject: Research
                Custom metadata
                issue-copyright-statement © The Author(s) 2021

                ScienceOpen disciplines: Biotechnology
                Keywords: mesenchymal stem cells (mscs),extracellular vesicles,osteoarthritis,circular rna hipk3 (circhipk3),mir-124-3p,myh9
                Data availability:
                ScienceOpen disciplines: Biotechnology
                Keywords: mesenchymal stem cells (mscs), extracellular vesicles, osteoarthritis, circular rna hipk3 (circhipk3), mir-124-3p, myh9

                Comments

                Comment on this article

                scite_

                Similar content408

                See all similar

                Cited by28

                See all cited by

                Most referenced authors921

                See all reference authors