Lifestyle exercise attenuates immunosenescence; flow cytometry analysis

Abstract

Background

Interaction of physical activity and overall immune profile is very complex and depends on the intensity, duration and frequency of undertaken physical activity, the exposure to cytomegalovirus (CMV) infection and the age-related changes in the immune system. Daily physical activity, which particularly influences immunity, declines dramatically with age. Therefore, the aim of the study was to explain whether physical activity sustained throughout life can attenuate or reverse immunosenescence.

Methods

Ninety-nine older adults (60–90 years) were recruited for the study. According to the 6-min walk test (6WMT), the Åstrand-Ryhming bike test (VO ₂max) and Community Healthy Activities Model Program for Seniors (CHAMPS) questionnaire, the individuals were classified as physically active ( n = 34) and inactive ( n = 20) groups. The analysis of T lymphocytes between active vs. inactive participants was performed using eight-parameter flow cytometry.

Results

Analysis of the baseline peripheral naïve and memory T lymphocytes showed a significant relationship of lifestyle exercise with the CD4/CD8 ratio. Above 50% of physically active participants demonstrated the CD4/CD8 ratio ≥ 1 or ≤ 2.5 contrary to the inactive group who showed the ratio < 1. The older adults with the result of 6WMT > 1.3 m/s and VO ₂max > 35 mL/kg/min had a significantly higher CD4 ⁺CD45RA ⁺ T lymphocyte percentage and also a higher ratio of CD4 ⁺CD45RA ⁺/CD4 ⁺CD45RO ⁺. Interestingly, in active older adults with IgG CMV ⁺ ( n = 30) the count of CD4 ⁺CD45RA ⁺ T lymphocytes was higher than in the inactive group with IgG CMV ⁺ ( n = 20).

Conclusion

Based on the flow cytometry analysis, we concluded that lifestyle exercise could lead to rejuvenation of the immune system by increasing the percentage of naïve T lymphocytes or by reducing the tendency of the inverse CD4/CD8 ratio.