Since their discovery, STAT proteins have been intimately tied to controlling the
development of hematopoietic cells that regulate inflammation, and mediating the responses
of target cells to inflammatory cytokines. Although our understanding of STAT protein-dependent
gene expression during inflammation has grown considerably over the past 15 years,
the identification of new cell types that require STAT proteins for development, and
new gene targets of STAT-dependent regulation, has added to the already complex network
of cells and cytokines in immune-mediated diseases. The review articles in this special
focus provide perspective on a number of areas of recent research and highlight some
of the next important questions in understanding how STAT proteins contribute to inflammatory
disease.
Inflammation can be broadly defined as the recruitment of lymphoid and myeloid cells
to a site of injury or infection. Cytokines are integral to the development of inflammation
and as a result, STAT proteins are critical mediators of immunity to pathogens, and
in the development of inflammatory disease.
1
Indeed, inflammation was one of the earliest biological functions associated with
STAT proteins, from the anti-viral functions of STAT1, to the polarized T helper cell
responses that required STAT4 and STAT6. In this special focus on STAT Signaling in
Inflammation, six review articles by leading researchers describe the importance of
STATs in immune responses, cellular development, and as targets for therapy of immune-mediated
diseases. Although there is considerable work on how STAT proteins impact adaptive
immunity, particularly in T helper cell development, less work has focused on innate
responses and the development and function of innate immune cells. The reviews in
this special focus highlight some of the important milestones in understanding how
STAT proteins regulate inflammation, and suggest some of the unanswered questions
remaining in the field.
STAT1 is activated by type I and type II interferons and promotes an anti-viral state
within stimulated cells. Mice deficient in STAT1 exhibit enhanced susceptibility to
viral infections, succumbing to lethal viremia at typically non-lethal doses of infection.
However, the IFN/STAT1 pathway is important for immune responses more broadly than
viral immunity. Rauch, Müller and Decker
2
review the many ways that the IFN/STAT1 pathway promotes inflammation, including inducing
expression of chemokines, regulating the differentiation and death of hematopoietic
cells and promoting production of reactive oxygen species and nitric oxide. They further
explore how IFN signaling cooperates with pattern recognition receptor signaling in
generating innate immune response to bacteria, and bacterial products during systemic
and localized inflammation. This is particularly important in the intestine where
the reciprocal interactions between the gut microbiota and host cells broadly impact
health and disease.
Chowdhury and Farrar
3
focus on STAT2, activated only by type I and type III IFN receptors. STAT2 is also
unique in its obligate “partner” status in heterodimerizing with STAT1. Yet, STAT2
provides a central role in anti-viral responses, conferring the ability of type I
and III IFNs to activate a set of genes distinct from those genes activated by IFNγ
and STAT1 homodimers. The authors describe how STAT2 contributes to inflammation,
and how STAT2 is now recognized as an important target of viral immune evasion during
infection. STAT2 is the most evolutionarily divergent among all STAT proteins, a feature
that likely contributes to the host range and susceptibility to viral infection. Although
STAT2 has not been studied as extensively as some of the other STATs, it remains a
fascinating nexus for host-pathogen interactions.
Kallal and Biron
4
focus on an additional, and seldom considered, aspect of STAT biology in anti-viral
responses. Following changes in the environment during an infection, expression patterns
of STAT proteins within the cell are altered, and as a consequence, the STAT proteins
activated by a cytokine, and thus the genes activated by that cytokine, can differ.
This finding demonstrates how the same cytokine may invoke different responses not
only from two distinct cell types, but also from one cell type in two distinct states,
such as resting and activated where the expression of STAT proteins has changed. The
most dramatic example is in NK cells where altered expression of STAT1 changes the
activation of STAT4, and the outcome of type I IFN stimulation. This paradigm provides
not only a framework for understanding temporal changes in cytokine responses, but
also a mechanism for cellular memory following exposure to an inflammatory environment.
In systems where STAT protein expression is considered to be a constant, it will be
important to extend these observations and determine how dynamic changes in STAT expression
contribute to multiple aspects of inflammation.
As part of their function in mediating cytokine signals, STAT proteins are also required
for cellular differentiation of immune effectors. STAT6 was the first STAT shown to
be required for differentiation of a T helper subset. STAT6-dependent Th2 development
contributes to a variety of inflammatory diseases, positively impacting allergic inflammation
and negatively affecting autoimmunity.
5
,
6
Subsequently, STAT4-dependent Th1 differentiation was shown to be required for autoimmunity
and inflammatory disease.
7
This model has expanded to fit the growing list of T helper subsets, and has adjusted
to the realization that multiple STAT proteins can contribute to a single T helper
subset phenotype, and one STAT protein can be required for the development of several
T helper subsets.
8
,
9
The requirement for STAT proteins in the development of effector phenotypes is also
observed in T regulatory cells. Mahmud, Manlove and Farrar
10
review the importance of the IL-2/STAT5 signaling pathway in the development and function
of T regulatory cells. Although IL-2 was initially identified as a T cell growth factor,
it is now established as important for the T regulatory arm of the immune response.
Mice deficient in IL-2, or that have T cells deficient in expression of IL-2R components
or STAT5 have impaired development of thymic-derived natural T regulatory cells (nTreg).
The IL-2/STAT5 pathway promotes stability and effector function in inducible Tregs
(iTreg). STAT5 confers at least some of its function by binding to regulatory elements
of the Foxp3 gene. The activation of other STATs in iTreg can impair their development
or function, re-iterating the paradigm that cells integrate multiple cytokine/STAT
signals.
T helper cells are not the only hematopoietic lineages that are developmentally impacted
by cytokines and STAT proteins. Huang, Li and Qi
11
provide a review of cytokine and STAT protein effects in the development of innate
myeloid and lymphoid effector cells. The authors first review the requirement for
cytokines and STAT proteins in the development of myeloid effector cells including
basophils, mast cells and eosinophils. STAT5 is of particular importance, not only
for development of myeloid cell types, but also in the regulation of cytokine production
from these cells, through mechanisms that can be distinct from regulation of cytokines
in T cells. The authors then discuss our developing understanding of innate lymphoid
cell (ILC) subsets. Although the requirement for STAT proteins in these cells is not
well documented, recent reports have begun to define transcription factors that are
involved in ILC subset development and cytokine expression. If STAT proteins are required
for ILCs, it may change the interpretation of results from inflammatory disease models
using mice that are globally or conditionally deficient in STAT proteins.
The association of STATs with specific types of inflammation makes them attractive
targets for therapy. The previous issue of JAK-STAT (volume 1, issue 4) had a series
of reviews on the treatment of cancer with various approaches to inhibition of the
STAT-signaling pathway. Although these approaches have been more extensively examined
in cancer models, some are now being tested as approaches for inflammatory disease.
Egwuagu and Larkin
12
explore approaches to inhibit STAT pathways that promote the development of CNS autoimmune
diseases. The authors discuss the advantages and disadvantages of targeting specific
STAT proteins in the development of autoimmune disease knowing that blocking one STAT
pathway and T helper subset might lead to increased development of another subset
of T helper cells. STAT3 is required for autoinflammatory disease and the authors
discuss several approaches to inhibiting STAT3 directly, inhibiting STAT3-activating
pathways, and inhibiting the function of the STAT3 target genes Rora and Rorc. Given
the broad functions of STAT3 and its targets, the effectiveness of these approaches
will need to be carefully tested in pre-clinical models and in patient studies.
As noted in many of these articles, STAT proteins function in multiple cell types,
and that parallel activity contributes to the development of disease. For example,
in the development of allergic inflammation (see cover images), STAT6 is required
not only in T cells, but in airway epithelial cells, smooth muscle cells, B cells,
macrophages and likely other cell types. Much of what we know about STAT6 function
has been modeled on function in lymphocytes, and it is likely that STAT6 targets very
different genes, and possibly even works through different kinetics with different
co-factors in various cell types. As our efforts progress to understand how STAT proteins
function in multiple cell types, and how best to therapeutically interfere with STAT-dependent
gene regulation, the next frontier will be modifying those responses to inhibit inflammatory
disease, but appropriately enhance immunity and immune memory to pathogens.
About Dr Mark H. Kaplan. Dr Kaplan received a BSc from the University of Windsor and
a PhD in Immunology and Microbiology from Wayne State University. After postdoctoral
training at the University of Texas Southwestern Medical Center and at the Harvard
University School of Public Health, he joined the faculty at the Indiana University
School of Medicine. He became the Director of Pediatric Pulmonary Basic Research in
the Department of Pediatrics, leading a research program in asthma, allergic disease
and pulmonary inflammation. His research focuses on transcription factors in the differentiation
of T helper subsets and their contribution to the development of immunity and disease.