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      Diferencias reológicas de la masa de trigo en líneas recombinantes. II. Relación con combinaciones de los Loci Glu-1 y Glu-3 Translated title: Rheological differences of wheat dough in recombinant lines. II. Relationship with Loci Glu-1 and Glu-3 combinations

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          Abstract

          Las gliadinas y gluteninas de alto y bajo peso molecular (GAPM y GBPM) son el componente genético que definen en cierta medida la fuerza y extensibilidad (reología) de la masa. El objetivo del presente trabajo fue evaluar el efecto de las combinaciones de GAPM y GBPM sobre la reología del gluten de 98 líneas F6, obtenidas de Rebeca F2000 × Verano S91 y Gálvez M87 × Bacanora T88, y los progenitores. Los genotipos se sembraron en Roque, estado de Guanajuato, en el ciclo primavera-verano 2008. En muestras de las harinas se evaluaron variables asociadas con la fuerza y la extensibilidad de masa en el mixógrafo (tiempo de amasado, estabilidad al amasado, tolerancia al sobreamasado) y el alveógrafo de Chopin (fuerza general de la masa, W y la relación tenacidad-extensibilidad, P/L). Para efectuar el análisis, las progenies se agruparon de acuerdo con combinaciones de GAPM y GBPM. En la cruza Rebeca F2000 × Verano S91, la combinación Glu-1: 2*, 17 +18, 5+10 con Glu-3: c, h, b se caracterizó por tener gluten medio fuerte y extensible, lo cual también caracterizó a la combinación de Rebeca F2000 (1, 17+18, 5+10, c, g, b). La combinación 2*, 17+18, 2+12, e, g, b de las líneas recombinantes similares a Verano S91 se agrupó como de gluten débil y extensible. Las combinaciones recombinantes derivadas de la cruza Gálvez M87 × Bacanora T88 se clasificaron como de gluten medio fuerte a fuerte y extensible por su W y P/L. La combinación 1, 17+18, 5+10 b, h, c, que corresponde a Gálvez M87, fue la de mayor fuerza de la masa. La combinación 1, 7+9, 5+10, c, j, c, mostró características favorables de gluten a pesar de tener la presencia de la translocación 1B/1R, lo cual indica que es posible aminorar su efecto negativo a través del fitomejoramiento. Se concluye que existen combinaciones específicas de GAPM y GBPM que favorecen la calidad (fuerza y extensibilidad) de la masa.

          Translated abstract

          Gliadins and glutenins of high and low molecular weight (HMWG and LMWG) are the genetic component, defining to certain degree dough strength and extensibility (rheology). The objective of the present study was to evaluate the effect of HMWG and LMWG combinations on gluten rheology of 98 F6 lines, obtained from Rebeca F2000 × Verano S91 and Gálvez M87 × Bacanora T88 and progenitors. The genotypes were sown at Roque, state of Guanajuato, in the spring-summer cycle 2008. In flour samples, variables associated with strength and extensibility of dough were assessed in mixograph (kneading time, kneading stability, tolerance to over-kneading) and Chopin alveograph (general strength of the dough, W and elasticity-extensibility relationship, P/L). In order to conduct analysis, progenies were grouped according to HMWG and LMWG combinations. In the cross Rebeca F2000 × Verano S91, combination Glu-1: 2*, 17+18, 5+10 with Glu-3: c, h, b was described by having half-strong and extensible gluten, which also described the combination of Rebeca F2000 (1, 17+18, 5+10, c, g, b). The combination 2*, 17+18, 2+12, e, g, b of the recombinant lines similar to Verano S91 were grouped as weak and extensible gluten. The recombinant combinations derived from the cross Gálvez M87 x Bacanora T88 were classified as of half-strong to strong and extensible gluten by its W and P/L. Combination 1, 17+18, 5+10 b, h, c, which corresponds to Gálvez M87 was the one of greatest dough strength. Combination 1, 7+9, 5+10, c, j, c, showed favorable gluten properties despite presence of translocation 1B/1R, which indicates that it is possible to reduce its negative effect through plant improvement. It is concluded that there are specific HMWG and LMWG combinations that favor dough quality (strength and extensibility).

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          Most cited references21

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          A simplified SDS—PAGE procedure for separating LMW subunits of glutenin

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            Composition of HMW and LMW Glutenin Subunits and Their Effects on Dough Properties, Pan Bread, and Noodle Quality of Chinese Bread Wheats

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              Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats

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                Author and article information

                Journal
                agro
                Agrociencia
                Agrociencia
                Colegio de Postgraduados (Texcoco, Estado de México, Mexico )
                1405-3195
                2521-9766
                September 2010
                : 44
                : 6
                : 631-641
                Affiliations
                [03] Texcoco Estado de México orgnameCentro Internacional de Mejoramiento de Maíz y Trigo
                [02] Chapingo Estado de México orgnameInstituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias orgdiv1Campo Experimental Valle de México espitia.eduardo@ 123456inifap.gob.mx
                [01] Montecillo Estado de México orgnameColegio de Postgraduados
                Article
                S1405-31952010000600003 S1405-3195(10)04400600003
                31668476-31df-4f4e-acaa-a51101bf38e3

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : July 2010
                : January 2010
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 22, Pages: 11
                Product

                SciELO Mexico

                Categories
                Ciencia de los alimentos

                combinaciones de gluteninas,Triticum aestivum L.,reología del gluten,glutenin combinations,gluten rheology

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