A video tracking based improvement of acute toxicity test onArtemia salina

We have designed a real-time computer vision system, the Multi-Worm Tracker (MWT), that can simultaneously quantify the behavior of dozens of Caenorhabditis elegans on a traditional petri plate at video rates. Three traditional behavioral paradigms are examined using this system: spontaneous movement on food, where the behavior changes over tens of minutes; chemotaxis, where turning events must be detected accurately to determine strategy; and habituation of response to tap, where the response is stochastic and changes over time. In each case, manual analysis or automated single-worm tracking would be tedious and time-consuming, but the MWT system allows rapid quantification of behavior with minimal human effort. Thus, this system will enablelarge scale forward and reverse genetic screens for complex behaviors.

This paper presents a detailed characterisation of locomotor behaviour of a single Drosophila fly freely walking in a small square arena. Locomotor activity is monitored by a video-tracking paradigm. Multiple parameters are extracted to construct the portrait of locomotor activity: the total distance moved, the number of episodes of activity and inactivity, the duration of activity, and the mean walking speed. To initiate a quantification of the fly's spatial walking movements, the number of passages in a virtual centre zone has also been determined. Moreover, to reveal the trajectory, as an index of fly's navigation ability, the turning angle, the angular velocity and the meander have been measured. Finally, we show that the number of episodes of inactivity as function of their duration follows a power law, while its counterpart, the episodes of activity does not, suggesting that the overall pattern of locomotor activity adheres to a fractal-like structure. Remarkably, the majority of these parameters are sexually dimorphic. This fine description of locomotor activity represents a new tool which will facilitate the study of the role of the different brain structures in the organisation of locomotor activity and the localisation of the fly's central pattern generator for locomotion and its motivational control.

The U.S. Environmental Protection Agency is evaluating methods to screen and prioritize large numbers of chemicals for developmental toxicity. We are exploring methods to detect developmentally neurotoxic chemicals using zebrafish behavior at 6 days of age. The behavioral paradigm simultaneously tests individual larval zebrafish under both light and dark conditions in a 96-well plate using a video tracking system. We have found that many variables affect the level or pattern of locomotor activity, including age of the larvae, size of the well, and the presence of malformations. Some other variables, however, do not appear to affect larval behavior including type of rearing solution (10% Hank's vs. 1:3 Danieau vs 60 mg/kg Instant Ocean vs 1× and 1:10× EPA Moderately Hard Water). Zebrafish larval behavior using a microtiter plate format may be an ideal endpoint for screening developmentally neurotoxic chemicals, but it is imperative that many test variables be carefully specified and controlled. Published by Elsevier Inc.