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      Perspectives for integrated insect pest protection in oilseed rape breeding

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          Abstract

          In the past, breeding for incorporation of insect pest resistance or tolerance into cultivars for use in integrated pest management schemes in oilseed rape/canola ( Brassica napus) production has hardly ever been approached. This has been largely due to the broad availability of insecticides and the complexity of dealing with high-throughput phenotyping of insect performance and plant damage parameters. However, recent changes in the political framework in many countries demand future sustainable crop protection which makes breeding approaches for crop protection as a measure for pest insect control attractive again. At the same time, new camera-based tracking technologies, new knowledge-based genomic technologies and new scientific insights into the ecology of insect– Brassica interactions are becoming available. Here we discuss and prioritise promising breeding strategies and direct and indirect breeding targets, and their time-perspective for future realisation in integrated insect pest protection of oilseed rape. In conclusion, researchers and oilseed rape breeders can nowadays benefit from an array of new technologies which in combination will accelerate the development of improved oilseed rape cultivars with multiple insect pest resistances/tolerances in the near future.

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          Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome.

          Oilseed rape (Brassica napus L.) was formed ~7500 years ago by hybridization between B. rapa and B. oleracea, followed by chromosome doubling, a process known as allopolyploidy. Together with more ancient polyploidizations, this conferred an aggregate 72× genome multiplication since the origin of angiosperms and high gene content. We examined the B. napus genome and the consequences of its recent duplication. The constituent An and Cn subgenomes are engaged in subtle structural, functional, and epigenetic cross-talk, with abundant homeologous exchanges. Incipient gene loss and expression divergence have begun. Selection in B. napus oilseed types has accelerated the loss of glucosinolate genes, while preserving expansion of oil biosynthesis genes. These processes provide insights into allopolyploid evolution and its relationship with crop domestication and improvement. Copyright © 2014, American Association for the Advancement of Science.
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            CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

            Enhanced agricultural production through innovative breeding technology is urgently needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas genome editing enable efficient targeted modification in most crops, thus promising to accelerate crop improvement. Here, we review advances in CRISPR/Cas9 and its variants and examine their applications in plant genome editing and related manipulations. We highlight base-editing tools that enable targeted nucleotide substitutions and describe the various delivery systems, particularly DNA-free methods, that have linked genome editing with crop breeding. We summarize the applications of genome editing for trait improvement, development of techniques for fine-tuning gene regulation, strategies for breeding virus resistance, and the use of high-throughput mutant libraries. We outline future perspectives for genome editing in plant synthetic biology and domestication, advances in delivery systems, editing specificity, homology-directed repair, and gene drives. Finally, we discuss the challenges and opportunities for precision plant breeding and its bright future in agriculture.
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              Biosynthesis, function and metabolic engineering of plant volatile organic compounds.

              Plants synthesize an amazing diversity of volatile organic compounds (VOCs) that facilitate interactions with their environment, from attracting pollinators and seed dispersers to protecting themselves from pathogens, parasites and herbivores. Recent progress in -omics technologies resulted in the isolation of genes encoding enzymes responsible for the biosynthesis of many volatiles and contributed to our understanding of regulatory mechanisms involved in VOC formation. In this review, we largely focus on the biosynthesis and regulation of plant volatiles, the involvement of floral volatiles in plant reproduction as well as their contribution to plant biodiversity and applications in agriculture via crop-pollinator interactions. In addition, metabolic engineering approaches for both the improvement of plant defense and pollinator attraction are discussed in light of methodological constraints and ecological complications that limit the transition of crops with modified volatile profiles from research laboratories to real-world implementation. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
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                Author and article information

                Contributors
                christian.obermeier@agrar.uni-giessen.de
                Journal
                Theor Appl Genet
                Theor Appl Genet
                TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
                Springer Berlin Heidelberg (Berlin/Heidelberg )
                0040-5752
                1432-2242
                16 March 2022
                16 March 2022
                2022
                : 135
                : 11
                : 3917-3946
                Affiliations
                [1 ]GRID grid.8664.c, ISNI 0000 0001 2165 8627, Department of Plant Breeding, , Justus Liebig University, ; Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany
                [2 ]GRID grid.10388.32, ISNI 0000 0001 2240 3300, Plant Breeding Department, , University of Bonn, ; Katzenburgweg 5, 53115 Bonn, Germany
                [3 ]GRID grid.13946.39, ISNI 0000 0001 1089 3517, Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, , Julius Kühn Institute, ; Koenigin-Luise-Str. 19, 14195 Berlin, Germany
                [4 ]GRID grid.9464.f, ISNI 0000 0001 2290 1502, Department of Applied Entomology, , University of Hohenheim, ; Otto-Sander-Straße 5, 70599 Stuttgart, Germany
                [5 ]GRID grid.7450.6, ISNI 0000 0001 2364 4210, Division of Agricultural Entomology, , University of Göttingen, ; Grisebachstr. 6, 37077 Göttingen, Germany
                [6 ]Insitute for Resistance Research and Stress Tolerance, Julius Kühn Insitute, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany
                Author notes

                Communicated by Rajeev K. Varshney.

                Author information
                http://orcid.org/0000-0001-5605-9106
                http://orcid.org/0000-0003-2701-7964
                http://orcid.org/0000-0002-6607-8463
                http://orcid.org/0000-0002-9639-3042
                http://orcid.org/0000-0001-9722-6513
                http://orcid.org/0000-0001-7985-4292
                http://orcid.org/0000-0001-6380-3773
                Article
                4074
                10.1007/s00122-022-04074-3
                9729155
                35294574
                2f9cd59d-43b4-4fc1-8f34-74bdc77002e8
                © The Author(s) 2022

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 2 December 2021
                : 1 March 2022
                Funding
                Funded by: FundRef http://dx.doi.org/10.13039/501100010771, Bundesanstalt für Landwirtschaft und Ernährung;
                Award ID: 2219NR153
                Award Recipient :
                Funded by: Justus-Liebig-Universität Gießen (3114)
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                © Springer-Verlag GmbH Germany, part of Springer Nature 2022

                Genetics
                Genetics

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