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Abstract
For anaerobic digestion processes nitrogen sources are poorly investigated although
they are known as possible process limiting factors (in the hydrolysis phase) but
also as a source for fermentations for subsequent methane production by methanogenic
archaea. In the present study different complex and defined nitrogen sources were
investigated in a lab-scale experiment in order to study their potential to build
up methane. The outcome of the study can be summarised as follows: from complex nitrogen
sources yeast extract and casamino acids showed the highest methane production with
approximately 600 ml methane per mole of nitrogen, whereas by the use of skim milk
no methane production could be observed. From defined nitrogen sources L-arginine
showed the highest methane production with almost 1400 ml methane per mole of nitrogen.
Moreover it could be demonstrated that the carbon content and therefore C/N-ratio
has only minor influence for the methane production from the used substrates.