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      Genetic Distances of Binary Pen Shell Atrina pectinata Populations

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          Abstract

          The seven oligonucleotides primers were consumed to produce the quantity of unique loci shared to each pen shell team (ULSEPT) and quantity of loci shared by the binary pen shell teams. 154 quantities of LSBPP, with a mediocre of 22.0 per primer, were noticed in the binary pen shell ( Atrina pectinata ) teams. 328 fragments were recognized in the pen shell team A (PSTA), and 257 in the pen shell team B (PSTB): 77 quantities of ULSEPT (23.48%) in the PSTA and 121 (47.08%) in the PSTB. The band-sharing amount (BS amount) between entity’s no. 01 and no. 05 was the highest (0.884) between the binary PSTs. The median band-sharing amount of entities in the PSTA (0.685±0.011) was higher than in those invented from the PSTB (0.640±0.009) ( p<0.05). The highest genetic distance presenting substantial molecular difference was between entities PECTINATA no. 06 and PECTINATA no. 04 (0.498). Through this study, it is possible a certain degree to contribute to increasing the cultivation of pen shells, conservation of species, protection of the natural environment, and preservation of ecosystems.

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          Random amplified polymorphic DNA analysis of genetically modified organisms.

          Randomly amplified polymorphic DNA (RAPD) was used to analyzed 78 samples comprises of certified reference materials (soya and maize powder), raw seeds (soybean and maize), processed food and animal feed. Combination assay of two arbitrary primers in the RAPD analysis enable to distinguish genetically modified organism (GMO) reference materials from the samples tested. Dendrogram analysis revealed 13 clusters at 45% similarity from the RAPD. RAPD analysis showed that the maize and soybean samples were clustered differently besides the GMO and non-GMO products.
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            Comparative Analysis of Two Populations of the Brittle Star Amphiura filiformis (Echinodermata: Ophiuroidea) with Different Life History Strategies Using RAPD Markers.

            Random amplified polymorphic DNA (RAPD) analysis was applied to individuals of Amphiura filiformis sampled from two geographic locations that have previously been reported to have different life history strategies. Of thirty-one 10-mer RAPD primers screened initially, four were chosen and used in a comparative analysis of A. filiformis individuals collected from Galway Bay (Ireland) and Concarneau Bay (France). The results show much variation within A. filiformis populations. Although there are some rare alleles particular to each population, overall the populations were not genetically differentiated with the methods employed. Analysis of molecular variance (AMOVA) showed that a minimum of 93% of genotypic variance occurred among individuals within populations. The statistical significance of this pattern was supported by permutation tests. F(ST) values were not significantly different from and UPGMA cluster analyses based on three distance metrics did not separate the two populations.
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              Analysis of genetic structure of blacklip abalone ( Haliotis rubra ) populations using RAPD, minisatellite and microsatellite markers

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                Author and article information

                Journal
                Dev Reprod
                Dev Reprod
                Dev. Reprod.
                dr
                Development & Reproduction
                Korean Society of Developmental Biology
                2465-9525
                2465-9541
                September 2022
                30 September 2022
                : 26
                : 3
                : 127-133
                Affiliations
                [1 ]Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University , Gunsan 54150, Korea
                Author notes
                [] Corresponding author Jong-Man Yoon, Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University, Gunsan 54150, Korea., Tel: +82-63-469-1887, E-mail: jmyoon@ 123456kunsan.ac.kr
                Author information
                https://orcid.org/0000-0003-2825-681X
                Article
                dr-26-3-127
                10.12717/DR.2022.26.3.127
                9578318
                36285150
                21f4c82b-9d2b-467e-81ea-40f448c9c891
                © Copyright 2022 The Korean Society of Developmental Biology

                This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 19 June 2022
                : 31 July 2022
                : 06 September 2022
                Categories
                Short Communication
                Custom metadata
                2022-09-30

                atrina pectinata,band-sharing amount (bs amount),genetic clusters (gcs),genetic distance (gd),oligonucleotides primers (oligo-primers),hierarchical circular dendrogram (hcdg)

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