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      Chronological reassessment of the Middle to Upper Paleolithic transition and Early Upper Paleolithic cultures in Cantabrian Spain

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          Abstract

          Methodological advances in dating the Middle to Upper Paleolithic transition provide a better understanding of the replacement of local Neanderthal populations by Anatomically Modern Humans. Today we know that this replacement was not a single, pan-European event, but rather it took place at different times in different regions. Thus, local conditions could have played a role. Iberia represents a significant macro-region to study this process. Northern Atlantic Spain contains evidence of both Mousterian and Early Upper Paleolithic occupations, although most of them are not properly dated, thus hindering the chances of an adequate interpretation. Here we present 46 new radiocarbon dates conducted using ultrafiltration pre-treatment method of anthropogenically manipulated bones from 13 sites in the Cantabrian region containing Mousterian, Aurignacian and Gravettian levels, of which 30 are considered relevant. These dates, alongside previously reported ones, were integrated into a Bayesian age model to reconstruct an absolute timescale for the transitional period. According to it, the Mousterian disappeared in the region by 47.9–45.1ka cal BP, while the Châtelperronian lasted between 42.6k and 41.5ka cal BP. The Mousterian and Châtelperronian did not overlap, indicating that the latter might be either intrusive or an offshoot of the Mousterian. The new chronology also suggests that the Aurignacian appears between 43.3–40.5ka cal BP overlapping with the Châtelperronian, and ended around 34.6–33.1ka cal BP, after the Gravettian had already been established in the region. This evidence indicates that Neanderthals and AMH co-existed <1,000 years, with the caveat that no diagnostic human remains have been found with the latest Mousterian, Châtelperronian or earliest Aurignacian in Cantabrian Spain.

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          Dealing with Outliers and Offsets in Radiocarbon Dating

          The wide availability of precise radiocarbon dates has allowed researchers in a number of disciplines to address chronological questions at a resolution which was not possible 10 or 20 years ago. The use of Bayesian statistics for the analysis of groups of dates is becoming a common way to integrate all of the14C evidence together. However, the models most often used make a number of assumptions that may not always be appropriate. In particular, there is an assumption that all of the14C measurements are correct in their context and that the original14C concentration of the sample is properly represented by the calibration curve.
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            Early dispersal of modern humans in Europe and implications for Neanderthal behaviour.

            The appearance of anatomically modern humans in Europe and the nature of the transition from the Middle to Upper Palaeolithic are matters of intense debate. Most researchers accept that before the arrival of anatomically modern humans, Neanderthals had adopted several 'transitional' technocomplexes. Two of these, the Uluzzian of southern Europe and the Châtelperronian of western Europe, are key to current interpretations regarding the timing of arrival of anatomically modern humans in the region and their potential interaction with Neanderthal populations. They are also central to current debates regarding the cognitive abilities of Neanderthals and the reasons behind their extinction. However, the actual fossil evidence associated with these assemblages is scant and fragmentary, and recent work has questioned the attribution of the Châtelperronian to Neanderthals on the basis of taphonomic mixing and lithic analysis. Here we reanalyse the deciduous molars from the Grotta del Cavallo (southern Italy), associated with the Uluzzian and originally classified as Neanderthal. Using two independent morphometric methods based on microtomographic data, we show that the Cavallo specimens can be attributed to anatomically modern humans. The secure context of the teeth provides crucial evidence that the makers of the Uluzzian technocomplex were therefore not Neanderthals. In addition, new chronometric data for the Uluzzian layers of Grotta del Cavallo obtained from associated shell beads and included within a Bayesian age model show that the teeth must date to ~45,000-43,000 calendar years before present. The Cavallo human remains are therefore the oldest known European anatomically modern humans, confirming a rapid dispersal of modern humans across the continent before the Aurignacian and the disappearance of Neanderthals. ©2011 Macmillan Publishers Limited. All rights reserved
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              AMS Radiocarbon Dating of Ancient Bone Using Ultrafiltration

              The Oxford Radiocarbon Accelerator Unit (ORAU) has used an ultrafiltration protocol to further purify gelatin from archaeological bone since 2000. In this paper, the methodology is described, and it is shown that, in many instances, ultrafiltration successfully removes low molecular weight contaminants that less rigorous methods may not. These contaminants can sometimes be of a different radiocarbon age and, unless removed, may produce erroneous determinations, particularly when one is dating bones greater than 2 to 3 half-lives of 14 C and the contaminants are of modern age. Results of the redating of bone of Late Middle and Early Upper Paleolithic age from the British Isles and Europe suggest that we may need to look again at the traditional chronology for these periods.
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                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: Funding acquisitionRole: InvestigationRole: MethodologyRole: Project administrationRole: ResourcesRole: SoftwareRole: SupervisionRole: ValidationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: Formal analysisRole: InvestigationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: ValidationRole: Writing – original draftRole: Writing – review & editing
                Role: InvestigationRole: Project administrationRole: Writing – original draftRole: Writing – review & editing
                Role: ResourcesRole: Writing – review & editing
                Role: ResourcesRole: Writing – review & editing
                Role: ResourcesRole: Writing – review & editing
                Role: ResourcesRole: Writing – review & editing
                Role: ResourcesRole: Writing – review & editing
                Role: Formal analysisRole: SoftwareRole: ValidationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                18 April 2018
                2018
                : 13
                : 4
                : e0194708
                Affiliations
                [1 ] Instituto Internacional de Investigaciones Prehistóricas de Cantabria, (Universidad de Cantabria, Santander, Gobierno de Cantabria), Santander, Spain
                [2 ] Leverhulme Centre for Evolutionary Studies, Department of Archaeology, University of Cambridge, Cambridge, United Kingdom
                [3 ] Centro Nacional de Investigación de la Evolución Humana, Burgos, Spain
                [4 ] Department of Anthropology, MSC01 1040, University of New Mexico, Albuquerque, New Mexico, United States of America
                [5 ] Departamento de Historia, Universidad de Oviedo, Oviedo, Spain
                [6 ] Department of Archaeology, Simon Fraser University, Burnaby, British Columbia, Canada
                [7 ] Centro de Conservación e Investigación de los Materiales Arqueológicos y Paleontológicos de Gipuzkoa, Donostia/San Sebastián, Spain
                [8 ] Department of Civil and Environmental Engineering, Imperial College London, London, United Kingdom
                New York State Museum, UNITED STATES
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                http://orcid.org/0000-0003-3353-5581
                Article
                PONE-D-17-29779
                10.1371/journal.pone.0194708
                5905894
                29668700
                21536da6-bcc6-4d70-beb8-6546020709c1
                © 2018 Marín-Arroyo et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 11 August 2017
                : 8 March 2018
                Page count
                Figures: 5, Tables: 1, Pages: 20
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/100011264, FP7 People: Marie-Curie Actions;
                Award ID: FP7-PEOPLE-2012-CIG (Ref: 322112)
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/501100003329, Ministerio de Economía y Competitividad;
                Award ID: HAR2012-33956
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/501100003329, Ministerio de Economía y Competitividad;
                Award ID: RYC-2011-00695
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/501100006365, Universidad de Cantabria;
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/501100003176, Ministerio de Educación, Cultura y Deporte;
                Award ID: CAS15_00054
                Award Recipient :
                This research has been supported by the European Commission through FP7-PEOPLE-2012-CIG (Ref: 322112), the Spanish Ministry of Economy and Competitiveness (HAR2012-33956 and RYC-2011-00695), Cantabria Campus International and University of Cantabria to ABMA. The Instituto Internacional de Investigaciones Prehistóricas de Cantabria is sponsored by the University of Cantabria, the Government of Cantabria and Banco Santander. This paper was mainly written during a research stay by one of us (ABMA) at the Leverhulme Centre for Evolutionary Studies, Department of Archaeology, University of Cambridge funded by Spanish Ministry of Education, Culture and Sports (Jose de Castillejo Program, Ref no. CAS15_00054). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
                Categories
                Research Article
                Social Sciences
                Archaeology
                Archaeological Dating
                Radioactive Carbon Dating
                Research and Analysis Methods
                Chemical Characterization
                Isotope Analysis
                Radioactive Carbon Dating
                Social Sciences
                Archaeology
                Archaeological Dating
                Social Sciences
                Archaeology
                Earth Sciences
                Geology
                Geologic Time
                Stone Age
                Paleolithic Period
                Social Sciences
                Anthropology
                Paleoanthropology
                Biology and Life Sciences
                Paleontology
                Paleoanthropology
                Earth Sciences
                Paleontology
                Paleoanthropology
                Social Sciences
                Anthropology
                Physical Anthropology
                Paleoanthropology
                Biology and Life Sciences
                Physical Anthropology
                Paleoanthropology
                Social Sciences
                Sociology
                Culture
                Biology and Life Sciences
                Biochemistry
                Proteins
                Collagens
                Research and Analysis Methods
                Separation Processes
                Filtration
                Ultrafiltration
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                All relevant data are within the paper and its Supporting Information files.

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