A novel jamming phase diagram links tumor invasion to non-equilibrium phase separation

Summary

It is well established that the early malignant tumor invades surrounding extracellular matrix (ECM) in a manner that depends upon material properties of constituent cells, surrounding ECM, and their interactions. Recent studies have established the capacity of the invading tumor spheroids to evolve into coexistent solid-like, fluid-like, and gas-like phases. Using breast cancer cell lines invading into engineered ECM, here we show that the spheroid interior develops spatial and temporal heterogeneities in material phase which, depending upon cell type and matrix density, ultimately result in a variety of phase separation patterns at the invasive front. Using a computational approach, we further show that these patterns are captured by a novel jamming phase diagram. We suggest that non-equilibrium phase separation based upon jamming and unjamming transitions may provide a unifying physical picture to describe cellular migratory dynamics within, and invasion from, a tumor.

Graphical abstract

Highlights

• •

  Tumor spheroids invading into a 3D matrix exhibit coexistent material phases

• •

  Invasion leads to spatiotemporal heterogeneities consistent with jamming dynamics

• •

  Phase separation patterns are captured by a model-based jamming phase diagram

• •

  Effective thermodynamic variables T _eff and P _conf capture cell and matrix properties

Abstract

Biophysics; Cancer; Mechanobiology