33
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Molecular prevalence and phylogeny of Anaplasma marginale, Anaplasma ovis and Theileria ovis in goats and sheep enrolled from a hill station in Punjab, Pakistan

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Anaplasma marginale ( A. marginale), Anaplasma ovis ( A. ovis) and Theileria ovis ( T. ovis) are among the most commonly reported intracellular tick borne pathogens that infect ruminants across the globe causing huge economic losses. This study aims to report the prevalence and phylogenetic evaluation of these three pathogens infecting sheep and goats (n = 333) that were enrolled from Fort Munro region in Pakistan by using msp1b, msp4 and 18S rRNA genes for A. marginale, A. ovis and T. ovis respectively. Results revealed almost similar infection rates in sheep and goats with an overall prevalence of 11% for A. marginale, 28% for A. ovis and 3% for T. ovis. Concurrent infection was also recorded, however, the number of animals infected with two pathogens (n = 24; 7.2%) was higher than infection with three pathogens (n = 2; 0.6%). Risk factor analysis revealed that sheep reared in small herds had higher A. marginale (P = 0.03) and A. ovis (P = 0.04) infection rates compared to those from large herds. In addition, it was observed that bucks (P ≤ 0.05) and tick-free goats (P ≤ 0.05) exhibited higher A. ovis infection rates than nannies. Phylogenetic analysis of all three pathogens showed that Pakistani isolates were clustered together and were closely related to previously deposited Pakistani isolates as well as with those that were reported from worldwide countries. In conclusion, we are reporting that Pakistani sheep and goats have A. marginale, A. ovis and T. ovis mediated infections and control measures should be taken against them to improve the productivity of the livestock sector.

          Related collections

          Most cited references47

          • Record: found
          • Abstract: found
          • Article: not found

          MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.

          The Molecular Evolutionary Genetics Analysis (Mega) software implements many analytical methods and tools for phylogenomics and phylomedicine. Here, we report a transformation of Mega to enable cross-platform use on Microsoft Windows and Linux operating systems. Mega X does not require virtualization or emulation software and provides a uniform user experience across platforms. Mega X has additionally been upgraded to use multiple computing cores for many molecular evolutionary analyses. Mega X is available in two interfaces (graphical and command line) and can be downloaded from www.megasoftware.net free of charge.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

            S Altschul (1997)
            The BLAST programs are widely used tools for searching protein and DNA databases for sequence similarities. For protein comparisons, a variety of definitional, algorithmic and statistical refinements described here permits the execution time of the BLAST programs to be decreased substantially while enhancing their sensitivity to weak similarities. A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original. In addition, a method is introduced for automatically combining statistically significant alignments produced by BLAST into a position-specific score matrix, and searching the database using this matrix. The resulting Position-Specific Iterated BLAST (PSI-BLAST) program runs at approximately the same speed per iteration as gapped BLAST, but in many cases is much more sensitive to weak but biologically relevant sequence similarities. PSI-BLAST is used to uncover several new and interesting members of the BRCT superfamily.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Anaplasma, Ehrlichia, and "Candidatus Neoehrlichia" bacteria: pathogenicity, biodiversity, and molecular genetic characteristics, a review.

              The family Anaplasmataceae includes gram-negative obligate intracellular alphaproteobacteria, which amplify within membrane-bound vacuoles. The genera Anaplasma and Ehrlichia as well as the recently specified cluster "Candidatus Neoehrlichia" comprise all bacteria of the family that are transmitted by ixodid ticks to mammalian hosts and cause infections in humans and numerous domestic and wild animal species. Totally, six Anaplasma, five Ehrlichia, and two "Candidatus Neoehrlichia" species as well as a variety of Ehrlichia bacteria with undefined taxonomic position belong to tick-borne bacteria. This review briefs the ecology, pathogenic properties, and molecular genetic diversity of Anaplasmataceae validated species, "Candidatus" species, and undefined novel Ehrlichia genetic variants. Copyright © 2011 Elsevier B.V. All rights reserved.
                Bookmark

                Author and article information

                Contributors
                Role: Formal analysis
                Role: Investigation
                Role: Formal analysis
                Role: Investigation
                Role: Formal analysis
                Role: Investigation
                Role: Investigation
                Role: Formal analysis
                Role: Methodology
                Role: MethodologyRole: SoftwareRole: Validation
                Role: Writing – original draft
                Role: ConceptualizationRole: Formal analysisRole: MethodologyRole: SupervisionRole: Writing – review & editing
                Role: ConceptualizationRole: Funding acquisitionRole: InvestigationRole: ResourcesRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS One
                plos
                PLOS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                8 November 2023
                2023
                : 18
                : 11
                : e0291302
                Affiliations
                [1 ] Department of Zoology, Ghazi University, Dera Ghazi Khan, Pakistan
                [2 ] Department of Zoology, Abdul Wali Khan University, Mardan, Pakistan
                [3 ] Department of Botany and Zoology, Bacha Khan University, Charsadda, Khyber Pakhtunkhwa, Pakistan
                [4 ] Institute of Zoology, Bahauddin Zakariya University, Multan, Pakistan
                [5 ] Department of Zoology, The Women University Multan, Pakistan
                [6 ] Department of Zoology, University of Sargodha, Pakistan
                [7 ] Department of Veterinary Medicine, University of Sassari, Sassari, Italy
                [8 ] Department of Pathology, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi, Taiwan
                [9 ] Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan
                [10 ] Ph.D. Program in Translational Medicine, Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan
                [11 ] Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan
                Cholistan University of Veterinary and Animal Sciences, Bahawalpur, PAKISTAN
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                https://orcid.org/0000-0001-5932-1982
                https://orcid.org/0000-0001-7852-9113
                https://orcid.org/0000-0003-4996-0152
                Article
                PONE-D-23-12196
                10.1371/journal.pone.0291302
                10631641
                37939034
                1d1820f0-4e31-4c0a-8363-39f1a78c2bff
                © 2023 Arif et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 26 April 2023
                : 22 August 2023
                Page count
                Figures: 4, Tables: 4, Pages: 16
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/501100012537, Ditmanson Medical Foundation Chia-Yi Christian Hospital;
                Award ID: R111-52
                Award Recipient :
                The article publication charges were funded by Ditmanson Medical Foundation Chia-Yi Christian Hospital through grant number R111-52 that was awarded to Prof. Dr. Chien-Chin Chen. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
                Categories
                Research Article
                Biology and Life Sciences
                Organisms
                Eukaryota
                Animals
                Vertebrates
                Amniotes
                Mammals
                Ruminants
                Sheep
                Biology and Life Sciences
                Zoology
                Animals
                Vertebrates
                Amniotes
                Mammals
                Ruminants
                Sheep
                Biology and Life Sciences
                Microbiology
                Medical Microbiology
                Microbial Pathogens
                Bacterial Pathogens
                Anaplasma
                Medicine and Health Sciences
                Pathology and Laboratory Medicine
                Pathogens
                Microbial Pathogens
                Bacterial Pathogens
                Anaplasma
                Biology and Life Sciences
                Organisms
                Bacteria
                Rickettsiales
                Anaplasma
                Biology and Life Sciences
                Parasitology
                Parasite Groups
                Apicomplexa
                Theileria
                Biology and Life Sciences
                Organisms
                Eukaryota
                Animals
                Vertebrates
                Amniotes
                Mammals
                Ruminants
                Biology and Life Sciences
                Zoology
                Animals
                Vertebrates
                Amniotes
                Mammals
                Ruminants
                Biology and Life Sciences
                Organisms
                Eukaryota
                Animals
                Vertebrates
                Amniotes
                Mammals
                Ruminants
                Goats
                Biology and Life Sciences
                Zoology
                Animals
                Vertebrates
                Amniotes
                Mammals
                Ruminants
                Goats
                Biology and Life Sciences
                Evolutionary Biology
                Evolutionary Systematics
                Phylogenetics
                Phylogenetic Analysis
                Biology and Life Sciences
                Taxonomy
                Evolutionary Systematics
                Phylogenetics
                Phylogenetic Analysis
                Computer and Information Sciences
                Data Management
                Taxonomy
                Evolutionary Systematics
                Phylogenetics
                Phylogenetic Analysis
                Medicine and Health Sciences
                Pathology and Laboratory Medicine
                Pathogens
                People and Places
                Geographical Locations
                Asia
                Pakistan
                Custom metadata
                All relevant data are within the manuscript and its Supporting information files.

                Uncategorized
                Uncategorized

                Comments

                Comment on this article

                scite_
                0
                0
                0
                0
                Smart Citations
                0
                0
                0
                0
                Citing PublicationsSupportingMentioningContrasting
                View Citations

                See how this article has been cited at scite.ai

                scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

                Similar content374

                Cited by3

                Most referenced authors1,218