In molecular biology, gene regulation is a fundamental biological process essential
to organisms. Generally, there are two broad levels of gene regulation: transcriptional
and post-transcriptional control. In gene regulation, the competing endogenous RNA
(ceRNA) regulation (Salmena et al., 2011) mediated by microRNAs (miRNAs) is one of
the most commonly studied mechanisms. At both transcriptional and post-transcriptional
levels, ceRNA regulation has been shown to be involved in many biological processes,
including the initiation and progression of human cancers (Tay et al., 2014). As a
novel layer of gene regulation, ceRNA regulation is higher than miRNA regulation in
terms of breadth, precision, and complexity (Smillie et al., 2018). Of the many types
of ceRNAs, the four most widely investigated are long non-coding RNAs (lncRNAs), pseudogenes,
circular RNAs (circRNAs), and messenger RNAs (mRNAs). Heretofore, numerous studies
(Tay et al., 2014; Qi et al., 2015; Wang et al., 2016; Misir et al., 2020) have revealed
that ceRNAs can act as potential diagnostic biomarkers in clinical applications.
In terms of cost, efficiency, and time consumption, computational methods are useful
to guide biological experiments in many areas of biology, and help us derive novel
biological insights (Lloyd, 2000; Editors of Nature Methods, 2021). With regard to
ceRNA regulation, computational methods have been demonstrated to greatly reduce the
time and cost of biological experiments (Le et al., 2017; Li et al., 2018; List et
al., 2019; Zhang et al., 2022). Novel computational methods or tools are being presented
to shortlist high-confidence ceRNAs for subsequent biological experiments. It is expected
that the development of computational methods or tools will drive novel biological
insights into the study of ceRNA regulation, and further speed up the research on
ceRNA (Figure 1).
FIGURE 1
Schema of exploring ceRNA regulation from multiple data sources.
This Research Topic of Frontiers in Molecular Biosciences features a collection of
Research articles on the computational or in silico identification of ceRNA regulation.
It is anticipated that this Research Topic will motivate researchers in the field
to accelerate their research on ceRNA and attempt to assist in subsequent experimental
design. Sabaie et al. applied a Positive Correlation (PC) method (Zhou et al., 2014;
Xu et al., 2015) to investigate the role of lncRNA-related ceRNAs in Autism Spectrum
Disorder (ASD), and found that four potential ceRNA axes (LINC00472/hsa-miR-221-3p/PTPN11,
ANP32A-IT1/hsa-miR-182-5p/S100A2, LINC00472/hsa-miR-132-3p/S100A2, and RBM26-AS1/hsa-miR-182-5p/S100A2)
may be involved in ASD pathogenesis. To understand the potential prognostic and immunological
roles of CCNA2 in pan-cancer, Chen et al. performed a pan-cancer analysis to identify
the upstream regulatory networks of CCNA2 and CCNA2-related ceRNAs in 33 tumor types.
Moreover, Guo et al. systematically analyzed and integrated chromosomal instability-related
dysregulated ceRNAs characteristics in lung adenocarcinoma (LUAD), and discovered
that the identified 12 dysregulated ceRNAs (AMOTL1, EFNB2, FGF2, FURIN, CCND2, IFNG,
ITGB4, RHOV, LINC00473, LINC00707, MIR497HG, and RP11-16E12.2) are closely associated
with multiple cancer progresses, especially immune-related pathways. In addition,
by integrating widely used computational methods and several public databases, Song
et al. developed an interactive R/Shiny tool, ceRNAshiny, for identification and analysis
of ceRNA regulation. Overall, these studies applied existing methods or developed
new tools to identify ceRNA regulation from bulk transcriptomics data, which provided
potential ceRNAs for subsequent biological experiments.
The explosive growth of biological data, especially omics data, provides opportunities
for computational biologists or bioinformaticians to develop methods or tools to unearth
biological implications hidden in the abundant data. Recently, although heterogeneous
data (e.g., omics and non-omics data) has opened a way to explore ceRNA regulation,
how to effectively integrate multiple data sources when developing novel computational
methods is still a challenge. Moreover, the identification of ceRNA regulation is
generally a computation-intensive task. For the fast inference of ceRNA regulation
in large-scale data, it is necessary to develop methods or tools with parallel computing.
Until now, existing computational methods are only confined to the study of ceRNA
regulation at the multi-sample level, rather than the ceRNA regulation at the single-sample
level. This may not precisely solve the heterogeneity of ceRNA regulation across individual
samples. Additionally, with the development and innovation of single-cell and spatial
sequencing technology, it will be an exciting direction to develop novel methods or
tools for exploring ceRNA regulation at the single-sample level. Finally, it is extremely
important to link ceRNA regulation with biological functions. However, how to connect
predicted ceRNA regulation with biological functions (e.g., human diseases) and establish
feasible benchmarks or guidelines for analyzing ceRNA regulation is still a challenge.
Altogether, to identify ceRNA regulation for assisting in subsequent experimental
design and discover potential ceRNA biomarkers for clinical application, developing
practical methods or tools is indispensable to the investigation of ceRNA regulation.