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      Screen-Printed Electrode-Based Sensors for Food Spoilage Control: Bacteria and Biogenic Amines Detection †

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          Abstract

          Food spoilage is caused by the development of microorganisms, biogenic amines, and other harmful substances, which, when consumed, can lead to different health problems. Foodborne diseases can be avoided by assessing the safety and freshness of food along the production and supply chains. The routine methods for food analysis usually involve long analysis times and complex instrumentation and are performed in centralized laboratories. In this context, sensors based on screen-printed electrodes (SPEs) have gained increasing importance because of their advantageous characteristics, such as ease of use and portability, which allow fast analysis in point-of-need scenarios. This review provides a comprehensive overview of SPE-based sensors for the evaluation of food safety and freshness, focusing on the determination of bacteria and biogenic amines. After discussing the characteristics of SPEs as transducers, the main bacteria, and biogenic amines responsible for important and common foodborne diseases are described. Then, SPE-based sensors for the analysis of these bacteria and biogenic amines in food samples are discussed, comparing several parameters, such as limit of detection, analysis time, and sample type.

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          Pathogenic Escherichia coli.

          Few microorganisms are as versatile as Escherichia coli. An important member of the normal intestinal microflora of humans and other mammals, E. coli has also been widely exploited as a cloning host in recombinant DNA technology. But E. coli is more than just a laboratory workhorse or harmless intestinal inhabitant; it can also be a highly versatile, and frequently deadly, pathogen. Several different E. coli strains cause diverse intestinal and extraintestinal diseases by means of virulence factors that affect a wide range of cellular processes.
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            Electrochemical biosensors.

            Electrochemical biosensors combine the sensitivity of electroanalytical methods with the inherent bioselectivity of the biological component. The biological component in the sensor recognizes its analyte resulting in a catalytic or binding event that ultimately produces an electrical signal monitored by a transducer that is proportional to analyte concentration. Some of these sensor devices have reached the commercial stage and are routinely used in clinical, environmental, industrial, and agricultural applications. The two classes of electrochemical biosensors, biocatalytic devices and affinity sensors, will be discussed in this critical review to provide an accessible introduction to electrochemical biosensors for any scientist (110 references).
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              The 12 principles of green analytical chemistry and the SIGNIFICANCE mnemonic of green analytical practices

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                Author and article information

                Journal
                Biosensors (Basel)
                Biosensors (Basel)
                biosensors
                Biosensors
                MDPI
                2079-6374
                30 September 2020
                October 2020
                : 10
                : 10
                : 139
                Affiliations
                [1 ]REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal; rdvdt@ 123456isep.ipp.pt (R.T.); han@ 123456isep.ipp.pt (H.P.A.N.)
                [2 ]Departamento de Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006 Oviedo, Spain
                Author notes
                [* ]Correspondence: costaestefania@ 123456uniovi.es (E.C.-R.); cmm@ 123456isep.ipp.pt (C.D.-M.)
                [†]

                This article is dedicated to the memory of Professor Agustín Costa-García.

                Author information
                https://orcid.org/0000-0002-5357-9269
                https://orcid.org/0000-0002-1472-8866
                https://orcid.org/0000-0002-3924-776X
                Article
                biosensors-10-00139
                10.3390/bios10100139
                7600659
                33008005
                193b5a6a-374c-4c7e-a70b-b37898398a05
                © 2020 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 31 August 2020
                : 28 September 2020
                Categories
                Review

                screen-printed electrode,electroanalysis,electrochemical sensor,biosensor,immunosensor,food analysis,bacteria,biogenic amines,histamine

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