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      DNA vaccines: designing strategies against parasitic infections

      review-article
      Author(s): 1 , 1 ,
      Publication date (Electronic):
      Journal: Genetic Vaccines and Therapy
      Publisher: BioMed Central

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          Abstract

          The complexity of parasitic infections requires novel approaches to vaccine design. The versatility of DNA vaccination provides new perspectives. This review discusses the use of prime-boost immunizations, genetic adjuvants, multivalent vaccines and codon optimization for optimal DNA vaccine design against parasites.

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          Most cited references52

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          Direct gene transfer into mouse muscle in vivo.

          Mark M. W. Chong, G Acsadi, Philip Felgner … (1990)
          RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for beta-galactosidase activity was localized to muscle cells following injection of the beta-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.
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            Enhanced T-cell immunogenicity of plasmid DNA vaccines boosted by recombinant modified vaccinia virus Ankara in humans.

            Tim Peto, Kent Kester, J. Smith … (2003)
            In animals, effective immune responses against malignancies and against several infectious pathogens, including malaria, are mediated by T cells. Here we show that a heterologous prime-boost vaccination regime of DNA either intramuscularly or epidermally, followed by intradermal recombinant modified vaccinia virus Ankara (MVA), induces high frequencies of interferon (IFN)-gamma-secreting, antigen-specific T-cell responses in humans to a pre-erythrocytic malaria antigen, thrombospondin-related adhesion protein (TRAP). These responses are five- to tenfold higher than the T-cell responses induced by the DNA vaccine or recombinant MVA vaccine alone, and produce partial protection manifest as delayed parasitemia after sporozoite challenge with a different strain of Plasmodium falciparum. Such heterologous prime-boost immunization approaches may provide a basis for preventative and therapeutic vaccination in humans.
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              The prime-boost strategy: exciting prospects for improved vaccination.

              Ian Ramshaw, Alistair J. Ramsay (2000)
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                Author and article information

                Journal
                Journal ID (nlm-ta): Genet Vaccines Ther
                Title: Genetic Vaccines and Therapy
                Publisher: BioMed Central (London )
                ISSN (Electronic): 1479-0556
                Publication date Collection: 2004
                Publication date (Electronic): 3 December 2004
                Volume: 2
                Page: 17
                Affiliations
                [1 ]Institute of Parasitology of McGill University, Macdonald Campus, 21,111 Lakeshore Road, Ste. Anne de Bellevue, Quebec, Canada, H9X 3V9
                Article
                Publisher ID: 1479-0556-2-17
                DOI: 10.1186/1479-0556-2-17
                PMC ID: 544584
                PubMed ID: 15579202
                SO-VID: 173cda95-c65e-4ee2-a62b-8bad921d4245
                Copyright © Copyright © 2004 Ivory and Chadee; licensee BioMed Central Ltd.
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 5 October 2004
                Date accepted : 3 December 2004
                Categories
                Subject: Review

                ScienceOpen disciplines: Genetics
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                ScienceOpen disciplines: Genetics

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