Microbial succession and pollutant removal metabolic pathways in anaerobic treatment of saline organic wastewater based on metagenomic technology

Many industrial sectors are likely to generate highly saline wastewater: these include the agro-food, petroleum and leather industries. The discharge of such wastewater containing at the same time high salinity and high organic content without prior treatment is known to adversely affect the aquatic life, water potability and agriculture. Thus, legislation is becoming more stringent and the treatment of saline wastewater, both for organic matter and salt removal, is nowadays compulsory in many countries. Saline effluents are conventionally treated through physico-chemical means, as biological treatment is strongly inhibited by salts (mainly NaCl). However, the costs of physico-chemical treatments being particularly high, alternative systems for the treatment of organic matter are nowadays increasingly the focus of research. Most of such systems involve anaerobic or aerobic biological treatment. Even though biological treatment of carbonaceous, nitrogenous and phosphorous pollution has proved to be feasible at high salt concentrations, the performance obtained depends on a proper adaptation of the biomass or the use of halophilic organisms. Another major limit is related to the turbidity problems inherent in saline effluents. For this reason, the major need for research in the future will be the combination of physico-chemical/biological treatment of saline industrial effluents, with regard to the global treatment chain, in order to meet the regulations. Show more

The conversion of biomass to CH4 (biomethanation) involves an anaerobic microbial food chain composed of at least three metabolic groups of which the first two decompose the complex biomass primarily to acetate, formate, and H2. The thermodynamics of these conversions are unfavorable requiring a symbiosis with the CH4-producing group (methanogens) that metabolize the decomposition products to favorable concentrations. The methanogens produce CH4 by two major pathways, conversion of the methyl group of acetate and reduction of CO2 coupled to the oxidation of formate or H2. This review covers recent advances in the fundamental understanding of both methanogenic pathways with the view of stimulating research towards improving the rate and reliability of the overall biomethanation process. Copyright © 2011 Elsevier Ltd. All rights reserved. Show more

Nitrate (NO3-) pollution in water and wastewater has become a serious global issue. Biological denitrification, which reduces NO3- to N2 (nitrogen gas) by denitrifying microorganisms, is an efficient and economical process for the removal of NO3- from water and wastewater. During the denitrification process, electron donor is required to provide electrons for reduction of NO3-. A variety of electron donors, including organic and inorganic compounds, can be used for denitrification. This paper reviews the state of the art of various electron donors used for biological denitrification. Depending on the types of electron donors, denitrification can be classified into heterotrophic and autotrophic denitrification. Heterotrophic denitrification utilizes organic compounds as electron donors, including low-molecular-weight organics (e.g. acetate, methanol, glucose, benzene, methane, etc.) and high-molecular-weight organics (e.g. cellulose, polylactic acid, polycaprolactone, etc.); while autotrophic denitrification utilizes inorganic compounds as electron donors, including hydrogen (H2), reduced sulfur compounds (e.g. sulfide, element sulfur and thiosulfate), ferrous iron (Fe2+), iron sulfides (e.g. FeS, Fe1-xS and FeS2), arsenite (As(Ш)) and manganese (Mn(II)). The biological denitrification processes and the representative denitrifying microorganisms are summarized based on different electron donors, and their denitrification performance, operating costs and environmental impacts are compared and discussed. The pilot- or full-scale applications were summarized. The concluding remarks and future prospects were provided. The biodegradable polymers mediated heterotrophic denitrification, as well as H2 and sulfur mediated autotrophic denitrification are promising denitrification processes for NO3- removal from various types of water and wastewater. Show more