Transcription factors MhDREB2A/MhZAT10 play a role in drought and cold stress response crosstalk in apple – ScienceOpen
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      Transcription factors MhDREB2A/MhZAT10 play a role in drought and cold stress response crosstalk in apple

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          Abstract

          Drought and cold stresses seriously affect tree growth and fruit yield during apple ( Malus domestica) production, with combined stress causing injury such as shoot shriveling. However, the molecular mechanism underlying crosstalk between responses to drought and cold stress remains to be clarified. In this study, we characterized the zinc finger transcription factor ZINC FINGER OF ARABIDOPSIS THALIANA 10 (ZAT10) through comparative analysis of shoot-shriveling tolerance between tolerant and sensitive apple rootstocks. MhZAT10 responded to both drought and cold stresses. Heterologous expression of MhZAT10 in the sensitive rootstock ‘G935' from domesticated apple ( Malus domestica) promoted shoot-shriveling tolerance, while silencing of MhZAT10 expression in the tolerant rootstock ‘SH6' of Malus honanensis reduced stress tolerance. We determined that the apple transcription factor DEHYDRATION RESPONSE ELEMENT-BINDING PROTEIN 2A (DREB2A) is a direct regulator activating the expression of MhZAT10 in response to drought stress. Apple plants overexpressing both MhDREB2A and MhZAT10 genes exhibited enhanced tolerance to drought and cold stress, while plants overexpressing MhDREB2A but with silenced expression of MhZAT10 showed reduced tolerance, suggesting a critical role of MhDREB2A-MhZAT10 in the crosstalk between drought and cold stress responses. We further identified drought-tolerant MhWRKY31 and cold-tolerant MhMYB88 and MhMYB124 as downstream regulatory target genes of MhZAT10. Our findings reveal a MhDREB2A-MhZAT10 module involved in crosstalk between drought and cold stress responses, which may have applications in apple rootstock breeding programs aimed at developing shoot-shriveling tolerance.

          Abstract

          The transcription factor MhDREB2A activates MhZAT10 expression, and they coordinately regulate drought and cold stress by integrating multiple pathways in apple rootstock to improve shoot-shriveling tolerance.

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          Most cited references69

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          Gene networks involved in drought stress response and tolerance.

          Plants respond to survive under water-deficit conditions via a series of physiological, cellular, and molecular processes culminating in stress tolerance. Many drought-inducible genes with various functions have been identified by molecular and genomic analyses in Arabidopsis, rice, and other plants, including a number of transcription factors that regulate stress-inducible gene expression. The products of stress-inducible genes function both in the initial stress response and in establishing plant stress tolerance. In this short review, recent progress resulting from analysis of gene expression during the drought-stress response in plants as well as in elucidating the functions of genes implicated in the stress response and/or stress tolerance are summarized. A description is also provided of how various genes involved in stress tolerance were applied in genetic engineering of dehydration stress tolerance in transgenic Arabidopsis plants.
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            Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.

            Full-length cDNAs are essential for functional analysis of plant genes in the post-sequencing era of the Arabidopsis genome. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. We have prepared a full-length cDNA microarray containing approximately 7000 independent, full-length cDNA groups to analyse the expression profiles of genes under drought, cold (low temperature) and high-salinity stress conditions over time. The transcripts of 53, 277 and 194 genes increased after cold, drought and high-salinity treatments, respectively, more than fivefold compared with the control genes. We also identified many highly drought-, cold- or high-salinity- stress-inducible genes. However, we observed strong relationships in the expression of these stress-responsive genes based on Venn diagram analysis, and found 22 stress-inducible genes that responded to all three stresses. Several gene groups showing different expression profiles were identified by analysis of their expression patterns during stress-responsive gene induction. The cold-inducible genes were classified into at least two gene groups from their expression profiles. DREB1A was included in a group whose expression peaked at 2 h after cold treatment. Among the drought, cold or high-salinity stress-inducible genes identified, we found 40 transcription factor genes (corresponding to approximately 11% of all stress-inducible genes identified), suggesting that various transcriptional regulatory mechanisms function in the drought, cold or high-salinity stress signal transduction pathways.
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              ABA-dependent and ABA-independent signaling in response to osmotic stress in plants.

              Plants have adaptive robustness to osmotic stresses such as drought and high salinity. Numerous genes functioning in stress response and tolerance are induced under osmotic conditions in diverse plants. Various signaling proteins, such as transcription factors, protein kinases and phosphatases, play signal transduction roles during plant adaptation to osmotic stress, with involvement ranging from stress signal perception to stress-responsive gene expression. Recent progress has been made in analyzing the complex cascades of gene expression during osmotic stress response, and especially in identifying specificity and crosstalk in abscisic acid (ABA)-dependent and ABA-independent signaling pathways. In this review, we highlight transcriptional regulation of gene expression governed by two key transcription factors: AREB/ABFs and DREB2A operating respectively in ABA-dependent and ABA-independent signaling pathways. Copyright © 2014 Elsevier Ltd. All rights reserved.
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                Author and article information

                Contributors
                Journal
                Plant Physiol
                Plant Physiol
                plphys
                Plant Physiology
                Oxford University Press (US )
                0032-0889
                1532-2548
                July 2023
                06 March 2023
                06 March 2023
                : 192
                : 3
                : 2203-2220
                Affiliations
                Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , Beijing 100093, People’s Republic of China
                Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , Beijing 100093, People’s Republic of China
                Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , Beijing 100093, People’s Republic of China
                Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , Beijing 100093, People’s Republic of China
                Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , Beijing 100093, People’s Republic of China
                Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , Beijing 100093, People’s Republic of China
                Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , Beijing 100093, People’s Republic of China
                Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , Beijing 100093, People’s Republic of China
                Author notes
                Author for correspondence: zhangjunke@ 123456baafs.net.cn

                The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors ( https://academic.oup.com/plphys/pages/general-instructions) is: Jun-Ke Zhang ( zhangjunke@ 123456baafs.net.cn ).

                Conflict of interest statement. The authors declare no conflict of interest.

                Author information
                https://orcid.org/0000-0002-2357-2058
                https://orcid.org/0000-0002-4580-4673
                https://orcid.org/0000-0002-1772-1264
                https://orcid.org/0000-0003-3802-5003
                https://orcid.org/0000-0002-1926-9574
                https://orcid.org/0000-0003-3873-4651
                https://orcid.org/0000-0003-2576-3728
                https://orcid.org/0000-0002-2991-4127
                Article
                kiad147
                10.1093/plphys/kiad147
                10315272
                36880407
                1243bc65-5728-4acc-ba63-feb8399786ef
                © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 18 November 2022
                : 15 February 2023
                : 22 March 2023
                Page count
                Pages: 18
                Funding
                Funded by: National Natural Science Foundation of China, DOI 10.13039/501100001809;
                Funded by: MARA;
                Categories
                Research Article
                Signaling and Response
                AcademicSubjects/SCI01270
                AcademicSubjects/SCI01280
                AcademicSubjects/SCI02286
                AcademicSubjects/SCI02287
                AcademicSubjects/SCI02288
                Plphys/50

                Plant science & Botany
                Plant science & Botany

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