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      Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)

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          Abstract

          We describe here the isolation and characterization of a major albumin from the seeds of Opuntia ficus-indica (Cactaceae). This protein has a molecular mass of 6.5 kDa and was isolated by a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of this protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The N-terminal amino acid sequence of this protein is Asp-Pro-Tyr-Trp-Glu-Gln-Arg.

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          A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

          M. Bradford (1976)
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            Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

            Hermann Schägger, Gebhard von Jagow (1987)
            A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations than in glycine-SDS-PAGE systems. A superior resolution of proteins, especially in the range between 5 and 20 kDa, is achieved without the necessity to use urea. Proteins above 30 kDa are already destacked within the sample gel. Thus a smooth passage of these proteins from sample to separating gel is warranted and overloading effects are reduced. This is of special importance when large amounts of protein are to be loaded onto preparative gels. The omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing.
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              Rapid analysis of amino acids using pre-column derivatization

              Brian Bidlingmeyer, Steven A. Cohen, Thomas L. Tarvin (1984)
              Article 0 comments Cited 175 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (publisher-id): bjmbr
                Title: Brazilian Journal of Medical and Biological Research
                Abbreviated Title: Braz J Med Biol Res
                Publisher: Associação Brasileira de Divulgação Científica (Ribeirão Preto, SP, Brazil )
                ISSN (Print): 0100-879X
                ISSN (Electronic): 1414-431X
                Publication date (Print and electronic): June 1998
                Volume: 31
                Issue: 6
                Pages: 757-761
                Affiliations
                [01] orgnameUniversidade Federal do Ceará orgdiv1 Departamento de Bioquímica e Biologia Molecular
                Article
                Publisher ID: S0100-879X1998000600005 Publisher ID: S0100-879X(98)03100605
                DOI: 10.1590/S0100-879X1998000600005
                SO-VID: 118cc79c-b146-4dad-8d79-9030e62d9ef9
                License:

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                Date accepted : 18 March 1998
                Date received : 29 September 1997
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 11, Pages: 5
                Product

                SciELO Brazil

                Categories
                Subject: Biochemistry and molecular biology

                Keywords: Cactaceae,2S albumins,seed proteins,Opuntia ficus-indica,prickly-pear
                Data availability:
                Keywords: Cactaceae, 2S albumins, seed proteins, Opuntia ficus-indica, prickly-pear

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