Rigor and reproducibility in human brain organoid research: Where we are and where we need to go

Summary

Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.

Graphical abstract

Abstract

Sandoval et al. reviewed the current quantitative methods used for cellular, molecular, and functional analyses of brain organoid models, with a focus on cortical organoids. The authors identified the challenges posed by this powerful and innovative technology and proposed recommendations to improve the reliability and reproducibility of data generated across laboratories.