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      • Record: found
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      • Article: found

      Antisense Basic Fibroblast Growth Factor Oligonucleotide Reduced Adhesion of Retinal Pigment Epithelial Cells to Extracellular Matrix Molecules and Their Proliferation

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          Abstract

          We investigated the effect of extracellular matrix molecules on the adhesion of retinal pigment epithelial cells and their subsequent proliferation. Fibronectin, collagen type I and vitronectin enhanced their adhesion and proliferation. In addition, the effect of basic fibroblast growth factor (bFGF) on their adhesion and proliferation was studied. bFGF enhanced their adhesion and proliferation, whereas antisense bFGF reduced their adhesion to and their proliferation on extracellular matrix molecules.

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          Expression of Fibroblast Growth Factors 1 and 2 in a Human Retinal Pigment Epithelium Cell Line (K1034)

          Daniela G Seidler, Johann S. Schwegler, Hans Liesenhoff (1999)
          The primary source of fibroblast growth factors (FGFs) is the retinal pigment epithelium (RPE). Investigations on FGF secretion in RPE primary cultures are hampered by the rapid run-down of cell vitality after a few passages. Therefore, long-term experiments require an alternative to primary cultures. We detected FGF-1 and FGF-2 in the established human K1034 cell line by immunohistochemistry. In addition, mRNA for both FGFs was found by RT-PCR. By immunohistochemistry, the signal was more pronounced with FGF-2 than with FGF-1. K1034 is capable of expressing both FGF-1 and FGF-2. With respect to these features, this cell line can be used as an alternative to primary cultured human RPE cells.
          Article 0 comments Cited 1 times – based on 0 reviews     Review now
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            Author and article information

            Journal
            Journal ID (publisher-id): ORE
            Journal ID (nlm-ta): Ophthalmic Res
            Journal ID (doi): 10.1159/issn.0030-3747
            Title: Ophthalmic Research
            Publisher: S. Karger AG
            ISSN (Print): 0030-3747
            ISSN (Electronic): 1423-0259
            Publication date Subscription-year: 2001
            Publication date (Print): February 2001
            Publication date (Electronic): 13 December 2000
            Volume: 33
            Issue: 1
            Pages: 24-30
            Affiliations
            Departments of aOphthalmology and bAnatomy and Cell Biology, Dong-A University College of Medicine and Institute of Medical Science, Pusan, South Korea
            Article
            Publisher ID: 55637 Other ID: Ophthalmic Res 2001;33:24–30
            DOI: 10.1159/000055637
            PubMed ID: 11114601
            SO-VID: 0df02cb5-0d1d-4cc7-846d-d27368d0e03d
            Copyright © © 2001 S. Karger AG, Basel
            License:

            Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

            History
            Page count
            Figures: 8, References: 21, Pages: 7
            Categories
            Subject: Original Paper

            ScienceOpen disciplines: Vision sciences,Ophthalmology & Optometry,Pathology
            Keywords: Proliferation,Adhesion,Antisense oligonucleotide,Basic fibroblast growth factor,Retinal pigment epithelium
            Data availability:
            ScienceOpen disciplines: Vision sciences, Ophthalmology & Optometry, Pathology
            Keywords: Proliferation, Adhesion, Antisense oligonucleotide, Basic fibroblast growth factor, Retinal pigment epithelium

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