5
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Mammalian cells exhibit remarkable diversity in cell size, but the factors that regulate establishment and maintenance of these sizes remain poorly understood. This is especially true for skeletal muscle, comprised of syncytial myofibers that each accrue hundreds of nuclei during development. Here, we directly explore the assumed causal relationship between multinucleation and establishment of normal size through titration of myonuclear numbers during mouse neonatal development. Three independent mouse models, where myonuclear numbers were reduced by 75, 55, or 25%, led to the discovery that myonuclei possess a reserve capacity to support larger functional cytoplasmic volumes in developing myofibers. Surprisingly, the results revealed an inverse relationship between nuclei numbers and reserve capacity. We propose that as myonuclear numbers increase, the range of transcriptional return on a per nuclear basis in myofibers diminishes, which accounts for both the absolute reliance developing myofibers have on nuclear accrual to establish size, and the limits of adaptability in adult skeletal muscle.

          Abstract

          Skeletal muscle is composed of syncytial myofibres, each containing hundreds of nuclei. Through genetic reduction of the number of nuclei per myofibre, the authors confirm that more nuclei produce larger cells but myofibres with fewer nuclei adaptively compensate leading to larger and functional myonuclear domains.

          Related collections

          Most cited references46

          • Record: found
          • Abstract: found
          • Article: not found

          Gene Ontology: tool for the unification of biology

          Genomic sequencing has made it clear that a large fraction of the genes specifying the core biological functions are shared by all eukaryotes. Knowledge of the biological role of such shared proteins in one organism can often be transferred to other organisms. The goal of the Gene Ontology Consortium is to produce a dynamic, controlled vocabulary that can be applied to all eukaryotes even as knowledge of gene and protein roles in cells is accumulating and changing. To this end, three independent ontologies accessible on the World-Wide Web (http://www.geneontology.org) are being constructed: biological process, molecular function and cellular component.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            PANTHER version 14: more genomes, a new PANTHER GO-slim and improvements in enrichment analysis tools

            Abstract PANTHER (Protein Analysis Through Evolutionary Relationships, http://pantherdb.org) is a resource for the evolutionary and functional classification of genes from organisms across the tree of life. We report the improvements we have made to the resource during the past two years. For evolutionary classifications, we have added more prokaryotic and plant genomes to the phylogenetic gene trees, expanding the representation of gene evolution in these lineages. We have refined many protein family boundaries, and have aligned PANTHER with the MEROPS resource for protease and protease inhibitor families. For functional classifications, we have developed an entirely new PANTHER GO-slim, containing over four times as many Gene Ontology terms as our previous GO-slim, as well as curated associations of genes to these terms. Lastly, we have made substantial improvements to the enrichment analysis tools available on the PANTHER website: users can now analyze over 900 different genomes, using updated statistical tests with false discovery rate corrections for multiple testing. The overrepresentation test is also available as a web service, for easy addition to third-party sites.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: found
              Is Open Access

              Heatmapper: web-enabled heat mapping for all

              Heatmapper is a freely available web server that allows users to interactively visualize their data in the form of heat maps through an easy-to-use graphical interface. Unlike existing non-commercial heat map packages, which either lack graphical interfaces or are specialized for only one or two kinds of heat maps, Heatmapper is a versatile tool that allows users to easily create a wide variety of heat maps for many different data types and applications. More specifically, Heatmapper allows users to generate, cluster and visualize: (i) expression-based heat maps from transcriptomic, proteomic and metabolomic experiments; (ii) pairwise distance maps; (iii) correlation maps; (iv) image overlay heat maps; (v) latitude and longitude heat maps and (vi) geopolitical (choropleth) heat maps. Heatmapper offers a number of simple and intuitive customization options for facile adjustments to each heat map's appearance and plotting parameters. Heatmapper also allows users to interactively explore their numeric data values by hovering their cursor over each heat map cell, or by using a searchable/sortable data table view. Heat map data can be easily uploaded to Heatmapper in text, Excel or tab delimited formatted tables and the resulting heat map images can be easily downloaded in common formats including PNG, JPG and PDF. Heatmapper is designed to appeal to a wide range of users, including molecular biologists, structural biologists, microbiologists, epidemiologists, environmental scientists, agriculture/forestry scientists, fish and wildlife biologists, climatologists, geologists, educators and students. Heatmapper is available at http://www.heatmapper.ca.
                Bookmark

                Author and article information

                Contributors
                douglas.millay@cchmc.org
                Journal
                Nat Commun
                Nat Commun
                Nature Communications
                Nature Publishing Group UK (London )
                2041-1723
                8 December 2020
                8 December 2020
                2020
                : 11
                : 6287
                Affiliations
                [1 ]GRID grid.239573.9, ISNI 0000 0000 9025 8099, Division of Molecular Cardiovascular Biology, , Cincinnati Children’s Hospital Medical Center, ; Cincinnati, OH 45229 USA
                [2 ]GRID grid.5510.1, ISNI 0000 0004 1936 8921, Department of Biosciences, , University of Oslo, ; Oslo, Norway
                [3 ]GRID grid.24827.3b, ISNI 0000 0001 2179 9593, Department of Internal Medicine, Division of Cardiovascular Health and Disease, , University of Cincinnati College of Medicine, ; Cincinnati, OH 45229 USA
                [4 ]GRID grid.5510.1, ISNI 0000 0004 1936 8921, Center for Integrative Neuroplasticity (CINPLA), Department of Biosciences, , University of Oslo, ; Oslo, Norway
                [5 ]GRID grid.13097.3c, ISNI 0000 0001 2322 6764, Center of Human and Applied Physiological Sciences, School of Basic and Medical Biosciences, Faculty of Life Sciences & Medicine, , King’s College London, ; London, UK
                [6 ]GRID grid.13097.3c, ISNI 0000 0001 2322 6764, Randall Center for Cell and Molecular Biophysics, School of Basic & Medical Biosciences, Faculty of Life Sciences & Medicine, Guy’s Campus, , King’s College London, ; London, UK
                [7 ]GRID grid.5254.6, ISNI 0000 0001 0674 042X, Department of Biomedical Sciences, , University of Copenhagen, ; Copenhagen, Denmark
                [8 ]GRID grid.24827.3b, ISNI 0000 0001 2179 9593, Department of Pediatrics, , University of Cincinnati College of Medicine, ; Cincinnati, OH 45229 USA
                Author information
                http://orcid.org/0000-0003-2997-5066
                http://orcid.org/0000-0003-3791-3287
                http://orcid.org/0000-0003-2006-7678
                http://orcid.org/0000-0002-6358-2920
                http://orcid.org/0000-0001-5188-0720
                Article
                20058
                10.1038/s41467-020-20058-7
                7722938
                33293533
                0ddfadab-9797-407a-8a49-02ac558aec74
                © The Author(s) 2020

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 11 December 2019
                : 30 October 2020
                Funding
                Funded by: FundRef https://doi.org/10.13039/100000049, U.S. Department of Health & Human Services | NIH | National Institute on Aging (U.S. National Institute on Aging);
                Award ID: R01AG059605
                Award Recipient :
                Funded by: FundRef https://doi.org/10.13039/100000069, U.S. Department of Health & Human Services | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS);
                Award ID: R01AR068286
                Award Recipient :
                Funded by: Pew Biomedical Scholar
                Categories
                Article
                Custom metadata
                © The Author(s) 2020

                Uncategorized
                developmental biology,skeletal muscle
                Uncategorized
                developmental biology, skeletal muscle

                Comments

                Comment on this article