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      Can the Density of Mineralized Dental Tissues (Dentin and Enamel) Be Measured and Compared with 3D Cone Beam Computed Tomography in Cases of Ectodermal Dysplasia?

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          Abstract

          Background

          Since 3-dimensional cone beam computed tomography (CBCT) started to be used in dentistry, mineral density can now be examined with computer software from the data on the images obtained. Detailed and clear images at different slice intervals can be obtained with CBCT, and mineral density can be measured from the image data on a computer with a Hounsfield unit (HU) scale. In addition to the broad opportunities presented by CBCT, this feature has presented a wider perspective to researchers.

          Material/Methods

          In this study, the CBCT images obtained from patients with the genetic disorder of ectodermal dysplasia were compared with the images of a control group to determine differences in mineralization of the teeth and to show that these could be compared by measuring the mineral density of dentin and enamel tissues using the HU scale on data from CBCT images. This opens new opportunities for cognitive and implementation research.

          Results

          In the study, CBCT images of 14 ectodermal dysplasia and 14 control group cases previously obtained for various reasons were used. Mineral density measurements were made from 4 different regions of the teeth of the ectodermal dysplasia and control groups (incisor edge of the crown, the center buccal, cervicale line, and apex of the teeth), and the groups were compared.

          Conclusions

          The aim of this study was to provide a new overview of the feasibility and suitability of mineralization measurement of dentin and enamel dental tissues with CBCT in ectodermal dysplasia and control groups.

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          Most cited references28

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          Mechanical properties of human dental enamel on the nanometre scale.

          Atomic force microscopy (AFM) combined with a nano-indentation technique was used to reveal the structure and to perform site-specific mechanical testing of the enamel of third molars. Nano-indentations (size<500 nm) were made in the cusp area to measure the mechanical properties of single enamel rods at different orientations. The influence of etching on the physical properties was studied and etching conditions that did not significantly alter the plastic-elastic response of enamel were defined. Elasticity and hardness were found to be a function of the microstructural texture. Mean Young's moduli of 87.5 (+/-2.2) and 72.2 (+/-4.5) GPa and mean hardness of 3.9+/-0.3 and 3.3+/-0.3 GPa were measured in directions parallel and perpendicular to the enamel rods, respectively. Analysis of variance showed that the differences were significant. The observed anisotropy of enamel is related to the alignment of fibre-like apatite crystals and the composite nature of enamel rods. Mechanical properties were also studied at different locations on single enamel rods. Compared to those in the head area of the rods, Young's moduli and hardness were lower in the tail area and in the inter-rod enamel, which can be attributed to changes in crystal orientation and the higher content of soft organic tissue in these areas.
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            Deriving Hounsfield units using grey levels in cone beam computed tomography.

            an in vitro study was performed to investigate the relationship between grey levels in dental cone beam CT (CBCT) and Hounsfield units (HU) in CBCT scanners. a phantom containing 8 different materials of known composition and density was imaged with 11 different dental CBCT scanners and 2 medical CT scanners. The phantom was scanned under three conditions: phantom alone and phantom in a small and large water container. The reconstructed data were exported as Digital Imaging and Communications in Medicine (DICOM) and analysed with On Demand 3D(R) by Cybermed, Seoul, Korea. The relationship between grey levels and linear attenuation coefficients was investigated. it was demonstrated that a linear relationship between the grey levels and the attenuation coefficients of each of the materials exists at some "effective" energy. From the linear regression equation of the reference materials, attenuation coefficients were obtained for each of the materials and CT numbers in HU were derived using the standard equation. HU can be derived from the grey levels in dental CBCT scanners using linear attenuation coefficients as an intermediate step.
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              Towards a new classification of ectodermal dysplasias.

              Ectodermal dysplasias (EDs) constitute a large and complex group of diseases characterized by various defects in hair, nails, teeth and sweat glands. Of the 170 EDs described so far, fewer than 30 have been explained at the molecular level with identification of the causative gene. This review proposes a new classification of EDs based on the function of the protein encoded by the mutated gene. The EDs are reviewed in light of the recent molecular and biochemical findings and an attempt is made to classify ED causative genes into four major functional subgroups: cell-cell communication and signalling; adhesion; transcription regulation; and development.
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                Author and article information

                Journal
                Med Sci Monit
                Med Sci Monit
                Medical Science Monitor
                Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
                International Scientific Literature, Inc.
                1234-1010
                1643-3750
                2022
                01 July 2022
                09 June 2022
                : 28
                : e937003-1-e937003-7
                Affiliations
                [1 ]Department of Restorative Dentistry, Faculty of Dentistry, Harran University, Sanlıurfa, Turkey
                [2 ]Department of Pediatric Dentistry, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey
                [3 ]Department of Pediatric Dentistry, Faculty of Dentistry, Harran University, Sanlıurfa, Turkey
                [4 ]Department of Public Health and Humanitarian Disciplines, Uzhhorod National University, Uzhhorod, Ukraine
                [5 ]Department of Biostatistics, Faculty of Medicine, Dicle University, Diyarbakir, Turkey
                Author notes
                Corresponding Author: Yasemin Yavuz, e-mail: yyavuz-21@ 123456hotmail.com
                [A]

                Study Design

                [B]

                Data Collection

                [C]

                Statistical Analysis

                [D]

                Data Interpretation

                [E]

                Manuscript Preparation

                [F]

                Literature Search

                [G]

                Funds Collection

                Article
                937003
                10.12659/MSM.937003
                9254724
                35773958
                0b990c81-e0c5-4f9f-a7c4-6d673ab7df2f
                © Med Sci Monit, 2022

                This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International ( CC BY-NC-ND 4.0)

                History
                : 20 April 2022
                : 27 May 2022
                Categories
                Clinical Research

                cbct,hounsfield units,mineral density,ectodermal dysplasia

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