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      Indispensability of the Escherichia coli carbonic anhydrases YadF and CynT in cell proliferation at a low CO2 partial pressure.

      Bioscience, biotechnology, and biochemistry
      Anion Transport Proteins, genetics, physiology, Bicarbonates, metabolism, Carbon Dioxide, Carbonic Anhydrases, Escherichia coli, enzymology, growth & development, Escherichia coli Proteins, Mutation, Partial Pressure

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          Abstract

          We found that a carbonic anhydrase, YadF, is essential for cell growth in the absence of another carbonic anhydrase, CynT, in Escherichia coli. However, mutant strains lacking both of them grew at high CO2 concentrations (5%), where non-enzymatic mechanisms generate HCO3-. This suggests that these carbonic anhydrases are essential because they maintain HCO3- levels at ambient CO2 concentrations.

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