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Abstract
To observe the effects of cinnamic acid (CINN) on the expression of tissue factor
(TF) induced by tumor necrosis factor alpha (TNFalpha) in human umbilical vein endothelium-derived
cell line (ECV304) and the related mechanisms.
Cellular TF activity was determined with 1-stage clotting assay in the presence or
absence of anti-TF antibody or DEGR-Vlla. TF mRNA was examined by semi-quantitative
reverse transcription polymerase chain reaction (RT-PCR). I-kappaB was measured by
Western blot analysis. Immunohistochemical analysis was performed to evaluate the
activation of NF-kappaB.
CINN (500 microg/mL) significantly decreased the TF activity in ECV304 induced by
TNFalpha (1000 U/mL) (p < 0.01) in a concentration-dependent fashion (r = -0.953,
p < 0.05). CINN also inhibited the expression of TF mRNA induced by TNFalpha. Immunohistochemical
analysis demonstrated NF-kappaB translocation from the cytoplasm to the nucleus, and
Western blot analysis showed I-KB decrement in cytoplasm after treatment of endothelial
cells with TNFalpha. CINN diminished these changes induced by TNFalpha.
CINN is able to attenuate TNFa-induced TF expression of vascular endothelial cells
by inhibiting NF-kappaB activation.