Evodiamine improves congnitive abilities in SAMP8 and APP ^swe/PS1 ^ΔE9 transgenic mouse models of Alzheimer's disease

Abstract

Aim:

To investigate the effect of evodiamine (a quinolone alkaloid from the fruit of Evodia rutaecarpa) on the progression of Alzheimer's disease in SAMP8 and APP ^swe/PS1 ^ΔE9 transgenic mouse models.

Methods:

The mice at age of 5 months were randomized into the model group, two evodiamine (50 mg·kg ⁻¹·d ⁻¹ and 100 mg·kg ⁻¹·d ⁻¹) groups and an Aricept (2 mg·kg ⁻¹·d ⁻¹) group. The littermates of no-transgenic mice and senescence accelerated mouse/resistance 1 mice (SAMR1) were used as controls. After 4 weeks of treatment, learning abilities and memory were assessed using Morris water-maze test, and glucose uptake by the brain was detected using positron emission tomography/computed tomography (PET/CT). Expression levels of IL-1β, IL-6, and TNF-α in brain tissues were detected using ELISA. Expression of COX-2 protein was determined using Western blot.

Results:

In Morris water-maze test, evodiamine (100 mg·kg ⁻¹·d ⁻¹) significantly alleviated the impairments of learning ability and memory. Evodiamine (100 mg·kg ⁻¹·d ⁻¹) also reversed the inhibition of glucose uptake due to development of Alzheimer's disease traits in mice. Furthermore, the dose of evodiamine significantly decreased the expression of IL-1β, IL-6, TNF-α, and COX-2 that were involved in the inflammation due to Alzheimer's disease.

Conclusion:

The results indicate that evodiamine (100 mg·kg ⁻¹·d ⁻¹) improves cognitive abilities in the transgenic models of Alzheimer's disease.