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      Cadáveres de caninos conservados con alcohol etílico y sal de cura y embalados al vacío para la enseñanza de la cirugía veterinaria Translated title: Dogs corpses preserved with ethyl alcohol and curing salt and vacuum-packed for teaching veterinary surgery

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          Abstract

          RESUMEN El objetivo de este trabajo fue analizar biomecánicamente la piel de cadáveres de caninos preparados químicamente con alcohol etílico y sal de cura, y embalados al vacío, para la práctica da cirugía veterinaria, además de obtener la evaluación microbiológica que se puede presentar durante el proceso. Se trabajó con ocho cadáveres de caninos, de peso 7.96±1.48 kg. Los animales fueron inyectados con 120 ml/kg de una solución de cloruro de sodio al 20%, nitrito al 1% y nitrato de sodio al 1%, y 150 ml/kg de alcohol con glicerina al 5% y se mantuvieron en envases al vacío a temperatura entre 0 y 4 °C. Se tomaron muestras de piel el día 0 (muestras frescas) y los días 30, 60, 90 y 120 para análisis biomecánico, así como para análisis microbiológico de los fluidos en los envases del embalaje plástico. La fuerza máxima de ruptura presentada por el grupo control y en los días de conservación demostró que la fijación con las sales de cura y el almacenamiento en embalajes al vacío mantuvo las características biomecánicas de la piel hasta por 120 días en los cadáveres bajo refrigeración.

          Translated abstract

          ABSTRACT The aim of this study was to biomechanically analyze the skin of canine corpses chemically prepared with ethyl alcohol and curing salt, and vacuum packed, for the practice of veterinary surgery, in addition to obtaining the microbiological evaluation that may occur during the process. Eight canine corpses, weighing 7.96 ± 1.48 kg, were used. The animals were injected with 120 ml/kg of a solution of 20% sodium chloride, 1% nitrite and 1% sodium nitrate, and 150 ml/kg of alcohol with 5% glycerin and kept in vacuum-plastic bags at temperature between 0 and 4 °C. Skin samples were taken on day 0 (fresh samples) and on days 30, 60, 90 and 120 for biomechanical analysis, as well as for microbiological analysis of the fluids in the plastic packaging containers. The maximum rupture force presented by the control group and in the days of conservation showed that the fixation with curimg salts and the storage in vacuum packs maintained the biomechanical characteristics of the skin for up to 120 days in the corpses under refrigeration.

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          Development and validation of a body condition score system for dogs

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            History and future of human cadaver preservation for surgical training: from formalin to saturated salt solution method.

            Traditionally, surgical training meant on-the-job training with live patients in an operating room. However, due to advancing surgical techniques, such as minimally invasive surgery, and increasing safety demands during procedures, human cadavers have been used for surgical training. When considering the use of human cadavers for surgical training, one of the most important factors is their preservation. In this review, we summarize four preservation methods: fresh-frozen cadaver, formalin, Thiel's, and saturated salt solution methods. Fresh-frozen cadaver is currently the model that is closest to reality, but it also presents myriad problems, including the requirement of freezers for storage, limited work time because of rapid putrefaction, and risk of infection. Formalin is still used ubiquitously due to its low cost and wide availability, but it is not ideal because formaldehyde has an adverse health effect and formalin-embalmed cadavers do not exhibit many of the qualities of living organs. Thiel's method results in soft and flexible cadavers with almost natural colors, and Thiel-embalmed cadavers have been appraised widely in various medical disciplines. However, Thiel's method is relatively expensive and technically complicated. In addition, Thiel-embalmed cadavers have a limited dissection time. The saturated salt solution method is simple, carries a low risk of infection, and is relatively low cost. Although more research is needed, this method seems to be sufficiently useful for surgical training and has noteworthy features that expand the capability of clinical training. The saturated salt solution method will contribute to a wider use of cadavers for surgical training.
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              Comparison of modified atmosphere packaging and vacuum packaging for long period storage of dry-cured ham: effects on colour, texture and microbiological quality.

              Slices of dry-cured hams (Biceps femoris muscle) were stored during 8 weeks under vacuum and modified atmospheres (100% N(2) and a mixture of 20% CO(2) and 80% N(2)) in order to study the modifications on colour, texture and microbial counts during that period. Lightness was found to be more stable when samples were stored with 20% CO(2) and 80% N(2) without statistical differences between vacuum and 100% N(2). A slight whiteness was observed in the vacuum packed samples. Yellowness increased during time in vacuum packed samples, although no differences were found among the three conditions at the end of the study. Redness values were not affected by time or by the packaging system. With regard to texture, values found for all samples were within the normal range for this type of products, although it was observed that modified atmosphere packaging preserved samples better from hardening than vacuum packaging. No safety problems were detected in relation to the microbial quality in any case. In general, no clear differences were found among the three packaging systems for colour, texture and microbial quality in the storage conditions studied.
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                Author and article information

                Journal
                rivep
                Revista de Investigaciones Veterinarias del Perú
                Rev. investig. vet. Perú
                Universidad Nacional Mayor de San Marcos. Facultad de Medicina Veterinaria (Lima, , Peru )
                1609-9117
                July 2021
                : 32
                : 4
                : e19075
                Affiliations
                [1] Jaboticabal São Paulo orgnameUniversidad Estadual de São Paulo (UNESP) orgdiv1Facultad de Ciencias Agrarias y Veterinarias orgdiv2Departamento de Morfología y Fisiología Animal Brazil
                [2] Jaboticabal São Paulo orgnameUniversidad Estadual de São Paulo (UNESP) orgdiv1Facultad de Ciencias Agrarias y Veterinarias orgdiv2Departamento de Patología Veterinaria Brazil
                Article
                S1609-91172021000400001 S1609-9117(21)03200400001
                10.15381/rivep.v32i4.19075
                0866c8ea-4faa-49ff-bb5f-a702f46862a0

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 09 November 2020
                : 30 April 2021
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 24, Pages: 0
                Product

                SciELO Peru

                Categories
                Artículos primarios

                teaching,anatomía,animal,cirugía,diéresis,sutura,enseñanza,anatomy,surgery,dieresis,suture

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