GEMINI: Initial behavioral results after full severance of the cervical spinal cord in mice

Abstract

Background:

The GEMINI spinal cord fusion protocol has been developed to achieve a successful cephalosomatic anastomosis. Here, we report the preliminary data on the use of a fusogen [polyethylene glycol (PEG)] after full cervical cord transection in mice to facilitate the fusion of both ends of a sharply transected spinal cord.

Methods:

Cervical laminectomy and a complete, visually confirmed cervical cord (C 5) transection was performed on female albino mice ( n = 16). In Group 1 ( n = 8), a fusogen, (PEG) was used to bridge the gap between the cut ends of the spinal cord. Group 2 received the same spinal cord transection but was treated with saline. Outcome was assessed daily using a standard scale (modified 22-point Basso-Beattie-Bresnahan scale) and filmed on camera.

Results:

The PEG group (group 1) showed partial restoration of motor function after 4 weeks of observation; group 2 (placebo) did not recover any useful motor activity.

Conclusion:

In this preliminary experiment, PEG, but not saline, promoted partial motor recovery in mice submitted to full cervical transection.