Geographic patterns of genomic diversity and structure in the C ₄ grass Panicum hallii across its natural distribution

Abstract

Geographic patterns of within-species genomic diversity are shaped by evolutionary processes, life history and historical and contemporary factors. New genomic approaches can be used to infer the influence of such factors on the current distribution of infraspecific lineages. In this study, we evaluated the genomic and morphological diversity as well as the genetic structure of the C ₄ grass Panicum hallii across its complex natural distribution in North America. We sampled extensively across the natural range of P. hallii in Mexico and the USA to generate double-digestion restriction-associated DNA (ddRAD) sequence data for 423 individuals from 118 localities. We used these individuals to study the divergence between the two varieties of P. hallii, P. hallii var. filipes and P. hallii var. hallii as well as the genetic diversity and structure within these groups. We also examined the possibility of admixture in the geographically sympatric zone shared by both varieties, and assessed distribution shifts related with past climatic fluctuations. There is strong genetic and morphological divergence between the varieties and consistent genetic structure defining seven genetic clusters that follow major ecoregions across the range. South Texas constitutes a hotspot of genetic diversity with the co-occurrence of all genetic clusters and admixture between the two varieties. It is likely a recolonization and convergence point of populations that previously diverged in isolation during fragmentation events following glaciation periods.

Abstract

Geographic patterns of diversity are shaped by historical and contemporary factors that drive evolutionary processes. Genomic approaches can be used to infer the influence of such factors on the current distribution of genetic variation. We studied diversity of the C ₄ grass Panicum hallii across its native range in North America. We observed strong genetic and morphological divergence across seven genetic clusters that follow major ecoregions. South Texas is a hotspot of genetic diversity with the co-occurrence of all genetic clusters and admixture between two described varieties. South Texas is likely a recolonization and convergence point of formerly diverged populations.