Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS

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Abstract

Quantitative information on blood metabolites can be used in developing advanced medical strategies such as early detection and prevention of disease. Monitoring bioactive lipids such as steroids, bile acids, and PUFA metabolites could be a valuable indicator of health status. However, a method for simultaneously measuring these bioactive lipids has not yet been developed. Here, we report a LC/MS/MS method that can simultaneously measure 144 bioactive lipids, including steroids, bile acids, and PUFA metabolites, from human plasma, and a sample preparation method for these targets. Protein removal by methanol precipitation and purification of bioactive lipids by solid-phase extraction improved the recovery of the targeted compounds in human plasma samples, demonstrating the importance of sample preparation methods for a wide range of bioactive lipid analyses. Using the developed method, we studied the plasma from healthy human volunteers and confirmed the presence of bioactive lipid molecules associated with sex differences and circadian rhythms. The developed method of bioactive lipid analysis can be applied to health monitoring and disease biomarker discovery in precision medicine.

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MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights

Zhiqiang Pang, Jasmine Chong, Guangyan Zhou … (2021)

Since its first release over a decade ago, the MetaboAnalyst web-based platform has become widely used for comprehensive metabolomics data analysis and interpretation. Here we introduce MetaboAnalyst version 5.0, aiming to narrow the gap from raw data to functional insights for global metabolomics based on high-resolution mass spectrometry (HRMS). Three modules have been developed to help achieve this goal, including: (i) a LC–MS Spectra Processing module which offers an easy-to-use pipeline that can perform automated parameter optimization and resumable analysis to significantly lower the barriers to LC-MS1 spectra processing; (ii) a Functional Analysis module which expands the previous MS Peaks to Pathways module to allow users to intuitively select any peak groups of interest and evaluate their enrichment of potential functions as defined by metabolic pathways and metabolite sets; (iii) a Functional Meta-Analysis module to combine multiple global metabolomics datasets obtained under complementary conditions or from similar studies to arrive at comprehensive functional insights. There are many other new functions including weighted joint-pathway analysis, data-driven network analysis, batch effect correction, merging technical replicates, improved compound name matching, etc. The web interface, graphics and underlying codebase have also been refactored to improve performance and user experience. At the end of an analysis session, users can now easily switch to other compatible modules for a more streamlined data analysis. MetaboAnalyst 5.0 is freely available at https://www.metaboanalyst.ca . Graphical Abstract From raw data to statistical and functional insights using MetaboAnalyst 5.0.

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PubChem Substance and Compound databases

Sunghwan Kim, Paul Thiessen, Evan Bolton … (2015)

PubChem (https://pubchem.ncbi.nlm.nih.gov) is a public repository for information on chemical substances and their biological activities, launched in 2004 as a component of the Molecular Libraries Roadmap Initiatives of the US National Institutes of Health (NIH). For the past 11 years, PubChem has grown to a sizable system, serving as a chemical information resource for the scientific research community. PubChem consists of three inter-linked databases, Substance, Compound and BioAssay. The Substance database contains chemical information deposited by individual data contributors to PubChem, and the Compound database stores unique chemical structures extracted from the Substance database. Biological activity data of chemical substances tested in assay experiments are contained in the BioAssay database. This paper provides an overview of the PubChem Substance and Compound databases, including data sources and contents, data organization, data submission using PubChem Upload, chemical structure standardization, web-based interfaces for textual and non-textual searches, and programmatic access. It also gives a brief description of PubChem3D, a resource derived from theoretical three-dimensional structures of compounds in PubChem, as well as PubChemRDF, Resource Description Framework (RDF)-formatted PubChem data for data sharing, analysis and integration with information contained in other databases.

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Prevalence and Clinical Manifestations of Primary Aldosteronism Encountered in Primary Care Practice.

Silvia Monticone, Jacopo Burrello, Davide Tizzani … (2017)

Despite being widely recognized as the most common form of secondary hypertension, among the general hypertensive population the true prevalence of primary aldosteronism (PA) and its main subtypes, aldosterone-producing adenoma (APA) and bilateral adrenal hyperplasia (BAH), remains a matter of debate.

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Author and article information

Contributors

Yoshihiro Izumi

Takeshi Bamba

Journal

Journal ID (nlm-ta): J Lipid Res

Journal ID (iso-abbrev): J Lipid Res

Title: Journal of Lipid Research

Publisher: American Society for Biochemistry and Molecular Biology

ISSN (Print): 0022-2275

ISSN (Electronic): 1539-7262

Publication date PMC-release: 20 December 2023

Publication date Collection: January 2024

Publication date (Electronic): 20 December 2023

Volume: 65

Issue: 1

Electronic Location Identifier: 100492

Affiliations

[1 ]Division of Metabolomics/Mass Spectrometry Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan

[2 ]Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

[3 ]Laboratory of Biomolecule Analysis, Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan

Author notes

[∗ ]For correspondence: Yoshihiro Izumi; Takeshi Bamba izumi@ 123456bioreg.kyushu-u.ac.jp bamba@ 123456bioreg.kyushu-u.ac.jp

Article

Publisher Item ID: S0022-2275(23)00165-7 Publisher ID: 100492

DOI: 10.1016/j.jlr.2023.100492

PMC ID: 10821590

PubMed ID: 38135255

SO-VID: 03a544d2-86bd-48bd-b1fa-eb674abbdf24

License:

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS

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Abstract

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Drug_transporters

Most cited references 35

MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights

PubChem Substance and Compound databases

Prevalence and Clinical Manifestations of Primary Aldosteronism Encountered in Primary Care Practice.

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