Vibrio paucivorans sp. nov. and Vibrio qingdaonensis sp. nov., two marine bacteria

Abstract

Two Gram-stain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacteria, designated strains DBSS07 ^T and ZSDZ65 ^T, were isolated from the surface sediments of the Bohai sea and Qingdao coastal seawater, respectively. Phylogenetic analysis based on 16S rRNA genes, multilocus sequence analysis (MLSA), phylogenomic analysis of single-copy gene families and whole-genome data placed DBSS07 ^T and ZSDZ65 ^T within the genus Vibrio . DBSS07 ^T was most closely related to Vibrio aestivus M22 ^T, with 97.51 % sequence similarity, whereas ZSDZ65 ^T was most closely related to Vibrio variabilis R-40492 ^T with 97.58 % sequence similarity. DBSS07 ^T grew with 1–7 % (w/v) NaCl (optimum 3 %), at 16–37 °C (optimum 28 °C) and at pH 6.0–9.0 (optimum pH 7.0); whereas ZSDZ65 ^T grew with 1–5 % (w/v) NaCl (optimum 2 %), at 16–32 °C (optimum 28 °C) and at pH 6.0–9.0 (optimum pH 8.0). Both strains shared the same major fatty acid components (more than 10 % of total fatty acids) of summed feature 3 (C _{16 : 1}ω7 c or/and C _{16 : 1}ω6 c), with different proportions. The DNA G+C contents of DBSS07 ^T and ZSDZ65 ^T were 44.7 and 44.3 %, respectively. On the basis of the results of polyphasic analysis, DBSS07 ^T and ZSDZ65 ^T are considered to represent novel species within the genus Vibrio , for which the names V. paucivorans sp. nov. (type strain, DBSS07 ^T = KCTC 82896 ^T= MCCC 1K06284 ^T) and V. qingdaonensis sp. nov. (type strain, ZSDZ65 ^T = KCTC 82893 ^T = MCCC 1K06289 ^T) are proposed, respectively.