Determination of the optimal enrichment Artemia franciscana with a synbiotic combination of probiotics Pediococcus acidilactici and prebiotic fructooligosaccharide

Abstract

In this study the optimal amount of enriching Artemia franciscana with a synbiotic combination of Pediococcus acidilactici and fructooligosaccharide (FOS( was investigated. The experiment was conducted in a completely randomized design in ten treatments: multi-level probiotics P. acidilactici (1×10 ⁹, 1×10 ⁸ and 1× 10 ⁷ CFU per L) and multi-level enriched prebiotic FOS (1, 2 and 5) g per L of solution and control. To evaluate the enrichment of adult artemia with each treatment, sampling was performed at 2, 4 and 6 hr after initiation of enrichment. The results showed that a synbiotic containing a probiotic treatments P. acidilactici at 1 × 10 ⁹ and 1 × 10 ⁸ CFU per L had more bacteria than a synbiotic containing a probiotic treatment with 1 × 10 ⁷ CFU per L ( p < 0.05), but did not show significant difference between levels of 1 × 10 ⁹ and 1 × 10 ⁸ CFU per L ( p > 0.05). The highest number of attached bacteria (6.78 ± 0.03 log CFU g ^-1) to adult artemia was shown after 6 hr of enrichment that showed significant difference with 2 hr, but did not show significant difference with 4 hr time. In conclusion, the results of this study showed that adult artemia in a short time (about 4 hr) unlike nauplii artemia can retain a large amount of probiotic (1 × 10 ⁸ CFU P. acidilactici per L and 5 g per L FOS prebiotic) on their own.