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      Effects of Coated Compound Proteases on Apparent Total Tract Digestibility of Nutrients and Apparent Ileal Digestibility of Amino Acids for Pigs

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          Abstract

          Two experiments were conducted to evaluate effects of coated compound proteases (CC protease) on apparent total tract digestibility (ATTD) of nitrogen (N) and energy, and apparent ileal digestibility (AID) of amino acids (AA) and nutrients in diets for pigs. In Exp. 1, 12 crossbred barrows (initial body weight: 20.14±1.71 kg) were housed in individual metabolism crates and allotted into 2 treatments with 6 piglets per treatment according to weight in a randomized complete block design. The 2 diets were corn-soybean meal basal diets with (0.2 g/kg) or without CC protease supplementation. The CC protease supplementation increased (p<0.05) the digestible and metabolizable N and energy values and the digestibility and retention rate of N in the diet. The ATTD of energy and nutrients had been improved (p<0.05) in the diet supplemented with CC protease. In Exp. 2, 12 crossbred barrows (initial body weight: 20.79±1.94 kg), fitted with T-cannulas at the distal ileum, were blocked by body weight into 2 groups with 6 pigs each. The diets were the same as those in Exp. 1. The CC protease increased (p<0.05) the AID of crude protein and some essential AA including arginine, isoleucine and leucine. The AID and ATTD of energy and nutrients had been improved (p<0.05) by supplemental CC protease, but the hindgut digestibility of nutrients was unaffected. Overall, the CC protease improved the ATTD of N and energy and AID of some indispensible AA and nutrients in the corn-soybean meal diet for pigs. Therefore, the CC protease supplement could improve the utilization of protein in the corn-soybean meal diet and thus contribute to lower N excretion to the environment.

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          Most cited references31

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          Nutrient Requirements of Swine : Eleventh Revised Edition

          (2012)
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            Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: terminology and application.

            In this review, the terminology that is used to describe the bioavailability and ileal digestibility of AA in pig feed ingredients is defined. Aspects of the methodology to establish bioavailability and ileal digestibility values also are discussed, and recommendations about the use of these values are provided. Two main factors can contribute to differences between bioavailability and ileal digestibility of AA. First, some AA, such as Lys, may be absorbed in chemical complexes that preclude their use for metabolism. Second, fermentation in the upper gut may result in a net loss or gain of AA to the animal. In addition, dietary effects on the efficiency of using bioavailable AA intake for tissue growth or milk production should be considered and may be attributed to endogenous AA losses in the hindgut and the metabolic costs associated with endogenous gut protein synthesis and losses. Ileal digestibility values may be expressed as apparent ileal digestibility (AID), standardized ileal digestibility (SID), or true ileal digestibility (TID). These terms are used to specify how ileal endogenous AA losses are reflected in digestibility values. Ileal endogenous AA losses may be separated into basal losses, which are not influenced by feed ingredient composition, and specific losses, which are induced by feed ingredient characteristics such as levels and types of fiber and antinutritional factors. Values for AID are established when total ileal outflow of AA (i.e., the sum of endogenous losses and nondigested dietary AA) is related to dietary AA intake. A concern with the use of AID values is that these are not additive in mixtures of feed ingredients. This concern may be overcome by correcting AID values for defined basal endogenous losses of AA, which yields SID values. Furthermore, if the AID values are corrected for basal and specific endogenous losses, then values for TID are calculated. However, reliable procedures to routinely measure specific endogenous losses are not yet available. It is recommended that basal ileal endogenous losses of AA should be measured in digestibility experiments using a defined protein-free diet and that these losses are reported with observed AID and SID values. It is suggested that SID values should be used for feed formulation, at least until more information on TID values becomes available.
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              Technical note: a technique for inserting a T-cannula into the distal ileum of pregnant sows.

              Our objective was to develop a technique for cannulating the terminal ileum in pregnant sows and to evaluate the usefulness of this procedure in digestibility studies in pregnant and lactating sows. A simple T-cannula was inserted into the terminal ileum approximately 15 cm cranial to the ileo-cecal valve in a total of 15 multiparous sows at d 40 (+/- 5 d) of pregnancy. All cannulated sows recovered quickly after the surgery and within 3 d they were eating normally. Elevated body temperatures were not registered in any sows, and clinical problems related to the surgery were not observed. At farrowing, normal litters were born, and number of stillborn pigs, number of live born pigs, and daily litter weight gain were not affected by the cannulations (P > .05). Of the 15 sows originally cannulated, 11 sows were used for collection of digesta during gestation and the following lactating period. Eight sows were rebred after weaning, and five sows were rebred after the second lactation period and kept for another cycle. Blockage of the cannulas never occurred, and no serious problems were associated with digesta collections. The experiment demonstrated that pregnant sows can be prepared with a simple T-cannula in the distal ileum and that the cannula can be maintained in sows throughout the reproductive cycle. Hence, the procedure provides a tool for obtaining digesta from pregnant and lactating sows for nutrition studies.
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                Author and article information

                Journal
                Asian-Australas J Anim Sci
                Asian-australas. J. Anim. Sci
                Asian-Australasian Journal of Animal Sciences
                Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
                1011-2367
                1976-5517
                December 2016
                31 December 2016
                : 29
                : 12
                : 1761-1767
                Affiliations
                State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre,China Agricultural University, Beijing 100193, China
                [1 ]Kemin Industries (Zhuhai) Co., Ltd., Zhuhai 519040, China
                Author notes
                [* ]Corresponding Author: X. S. Piao. Tel: +86-10-62733588, Fax: +86-10-62733688, E-mail: piaoxsh@ 123456cau.edu.cn

                State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University, Beijing 100193, China

                Article
                ajas-29-12-1761
                10.5713/ajas.16.0041
                5088425
                27004811
                70e70dd2-6b91-4d25-ae86-c7af3e60763d
                Copyright © 2016 by Asian-Australasian Journal of Animal Sciences

                This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 14 January 2016
                : 02 March 2016
                : 17 March 2016
                Categories
                Articles

                amino acid,apparent ileal digestibility,apparent total tract digestibility,coated compound proteases,nitrogen,pigs

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