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      Laboratory Diagnostics and Quality of Blood Collection.

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          Abstract

          Diagnostic blood samples collected by phlebotomy are the most common type of biological specimens drawn and sent to laboratory medicine facilities for being analyzed, thus supporting caring physicians in patient diagnosis, follow-up and/or therapeutic monitoring. Phlebotomy, a relatively invasive medical procedure, is indeed critical for the downstream procedures accomplished either in the analytical phase made in the laboratory or in the interpretive process done by the physicians. Diagnosis, management, treatment of patients and ultimately patient safety itself can be compromised by poor phlebotomy quality. We have read with interest a recent article where the authors addressed important aspects of venous blood collection for laboratory medicine analysis. The authors conducted a phlebotomy survey based on the Clinical and Laboratory Standard Institute (CLSI) H03-A6 document (presently replaced by the GP41-A6 document) in three government hospitals in Ethiopia to evaluate 120 professionals (101 non-laboratory professionals vs. 19 laboratory professionals) as regards the venous blood collection practice. The aim of this mini (non-systematic) review is to both take a cue from the above article and from current practices we had already observed in other laboratory settings, and discuss four questionable activities performed by health care professionals during venous blood collection. We refer to: i) diet restriction assessment; ii) puncture site cleansing; iii) timing of tourniquet removal and; iv) mixing specimen with additives.

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          Most cited references38

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          Lipemia: causes, interference mechanisms, detection and management

          In the clinical laboratory setting, interferences can be a significant source of laboratory errors with potential to cause serious harm for the patient. After hemolysis, lipemia is the most frequent endogenous interference that can influence results of various laboratory methods by several mechanisms. The most common preanalytical cause of lipemic samples is inadequate time of blood sampling after the meal or parenteral administration of synthetic lipid emulsions. Although the best way of detecting the degree of lipemia is measuring lipemic index on analytical platforms, laboratory experts should be aware of its problems, like false positive results and lack of standardization between manufacturers. Unlike for other interferences, lipemia can be removed and measurement can be done in a clear sample. However, a protocol for removing lipids from the sample has to be chosen carefully, since it is dependent on the analytes that have to be determined. Investigation of lipemia interference is an obligation of manufacturers of laboratory reagents; however, several literature findings report lack of verification of the declared data. Moreover, the acceptance criteria currently used by the most manufacturers are not based on biological variation and need to be revised. Written procedures for detection of lipemia, removing lipemia interference and reporting results from lipemic samples should be available to laboratory staff in order to standardize the procedure, reduce errors and increase patient safety.
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            Protons in ischemia: where do they come from; where do they go to?

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              Nursing blood specimen collection techniques and hemolysis rates in an emergency department: analysis of venipuncture versus intravenous catheter collection techniques.

              Re-collection of hemolyzed blood specimens delays patient care in overcrowded emergency departments. Our emergency department was unable to meet a benchmark of a 2% hemolysis rate for the collection of blood samples. Our hypothesis was that hemolysis rates of blood specimens differ dependent on the blood collection technique by venipuncture or intravenous catheter draw.
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                Author and article information

                Journal
                J Med Biochem
                Journal of medical biochemistry
                Walter de Gruyter GmbH
                1452-8258
                1452-8266
                Jul 2015
                : 34
                : 3
                Affiliations
                [1 ] Laboratory of Clinical Biochemistry, Department of Life and Reproduction Sciences, University of Verona, Italy; Post-Graduate Program of Pharmaceutical Sciences, Department of Medical Pathology, Federal University of Parana, Curitiba, Parana, Brazil; CAPES Foundation, Ministry of Education of Brazil, Brasília - DF, Brazil; MERCOSUL: Sector Committee of Clinical Analyses and In Vitro Diagnostics - CSM 20, Rio de Janeiro, Brazil.
                [2 ] Laboratory of Clinical Chemistry and Hematology, Academic Hospital of Parma, Parma, Italy.
                [3 ] Laboratory of Clinical Biochemistry, Department of Life and Reproduction Sciences, University of Verona, Italy.
                [4 ] Post-Graduate Program of Pharmaceutical Sciences, Department of Medical Pathology, Federal University of Parana, Curitiba, Parana, Brazil.
                [5 ] Laboratory of Clinical Biochemistry, Department of Life and Reproduction Sciences, University of Verona, Italy; Post-Graduate Program of Pharmaceutical Sciences, Department of Medical Pathology, Federal University of Parana, Curitiba, Parana, Brazil.
                Article
                jomb-2014-0043
                10.2478/jomb-2014-0043
                4922344
                28356839
                f2e5e747-aaa5-4ecc-9d78-6be4cb57df7b
                History

                blood specimen collection,tourniquet,specimen handling,phlebotomy,patient safety,medical errors

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