A serological assay to detect SARS-CoV-2 seroconversion in humans.

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Abstract

Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.

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Journal

Journal ID (iso-abbrev): Nat Med

Title: Nature medicine

Publisher: Springer Science and Business Media LLC

ISSN (Electronic): 1546-170X

ISSN (Print): 1078-8956

Publication date (Electronic): July 2020

Volume: 26

Issue: 7

Affiliations

[1 ] Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[2 ] Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[3 ] Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.

[4 ] Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia.

[5 ] Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[6 ] Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[7 ] Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[8 ] Division of Pulmonary, Critical Care, and Sleep Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[9 ] Department of Medicine, Mount Sinai Queens, Astoria, NY, USA.

[10 ] Division of Hospital Medicine, Mount Sinai Health System, New York, NY, USA.

[11 ] Division of Clinical Immunology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[12 ] Department of Pediatrics, the Icahn School of Medicine at Mount Sinai, NY, USA.

[13 ] Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, USA.

[14 ] Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[15 ] Travel Medicine Program, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

[16 ] School of Public Health and Preventive Medicine, Monash University and Infection Prevention and Healthcare Epidemiology Unit Alfred Health, Melbourne, Victoria, Australia.

[17 ] Department of Virology, Medicum, University of Helsinki, Helsinki, Finland.

[18 ] Veterinary Biosciences, Veterinary Faculty, University of Helsinki, Helsinki, Finland.

[19 ] Department of Virology and Immunology, Helsinki University Hospital (HUSLAB), Helsinki, Finland.

[20 ] Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland.

[21 ] Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. florian.krammer@mssm.edu.

Article

Publisher Item ID: 10.1038/s41591-020-0913-5 Mid ID: NIHMS1709279

DOI: 10.1038/s41591-020-0913-5

PMC ID: 8183627

PubMed ID: 32398876

SO-VID: 9cb6df0a-e076-49e5-9457-559161f8fa6e

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