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      Side-by-side Comparison of Skin Biopsies and Skin Tape Stripping Highlights Abnormal Stratum Corneum in Atopic Dermatitis

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          Abstract

          Skin biopsies are commonly used for the assessment of skin pathology in various skin diseases including atopic dermatitis (AD). However, due to the invasive nature of skin biopsies, many patients, particularly children, decline participation. This can lead to potential subject sampling bias as data could be skewed toward more severe, older patients willing to give biopsies. Recently, researchers have began studying the skin with a minimal, non-invasive technique using skin tape strips (STS) to profile the epidermal transcriptome, proteins, and lipids in the skin. However, side by side comparisons of skin biopsy and STS have not been done to assess epidermal penetration. Therefore, 20 STS were collected from the volar surface of forearm from healthy non-atopic subjects and patients with AD, followed by the collection of skin biopsies from adjacent non-taped and taped area of skin. Using H&E staining and immunostaining, we demonstrated that 20 STS reach the upper granular layer of the epidermis. Additionally, we found that the expression of terminal differentiation markers in samples from STS procedure positively correlated with the expression level of these markers in matching skin biopsies. Therefore, STS is a non-invasive and reliable approach to evaluate the expression of skin terminal differentiation markers, which are defective in AD skin.

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          Author and article information

          Journal
          0426720
          4839
          J Invest Dermatol
          J. Invest. Dermatol.
          The Journal of investigative dermatology
          0022-202X
          1523-1747
          7 June 2019
          06 June 2019
          November 2019
          01 November 2020
          : 139
          : 11
          : 2387-2389.e1
          Affiliations
          [1 ]Department of Pediatrics, National Jewish Health, Denver, CO 80206
          [2 ]Clinical Translational Research Center, National Jewish Health, CO 80206
          Author notes

          AUTHORSHIP CONDITIONS

          BEK and DYML initiated and designed studies. CB and PT collected tape samples and took skin biopsies from human subjects. PSK and KN extracted RNA from tape discs and performed real time RT-PCR. BEK and PSK performed immunostainings. BEK, EG and DYML analyzed data, prepared figures, and wrote the manuscript. All authors discussed the results and commented on the manuscript.

          [3 ]Address correspondence to: Donald Y.M. Leung, MD, PhD, National Jewish Health, 1400 Jackson St., Denver, CO 80206, TEL: 303-398-1379, FAX: 303-270-2182, Leungd@ 123456njhealth.org
          Article
          PMC6814531 PMC6814531 6814531 nihpa1531243
          10.1016/j.jid.2019.03.1160
          6814531
          31176708
          72e798f2-6385-4c99-b813-cc848b60db23
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