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Abstract
A new method was developed to acquire images automatically at a series of specimen
tilts, as required for tomographic reconstruction. The method uses changes in specimen
position at previous tilt angles to predict the position at the current tilt angle.
Actual measurement of the position or focus is skipped if the statistical error of
the prediction is low enough. This method allows a tilt series to be acquired rapidly
when conditions are good but falls back toward the traditional approach of taking
focusing and tracking images when necessary. The method has been implemented in a
program, SerialEM, that provides an efficient environment for data acquisition. This
program includes control of an energy filter as well as a low-dose imaging mode, in
which tracking and focusing occur away from the area of interest. The program can
automatically acquire a montage of overlapping frames, allowing tomography of areas
larger than the field of the CCD camera. It also includes tools for navigating between
specimen positions and finding regions of interest.