3
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Manuka honey: A promising wound dressing material for the chronic nonhealing discharging wounds: A retrospective study

      research-article

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Objectives:

          To assess the efficacy and feasibility of topical manuka honey application in chronic nonhealing discharging extraoral wounds.

          Materials and Methods:

          The study includes 15 patients (9 males and 6 females, mean age: 38.06, range: 20–50 years), presenting with the complaint of chronic nonhealing discharging extraoral wounds from January 2018 to January 2020. After wound irrigation with normal saline, manuka honey in conjunction with the antibiotic treatment was directly applied onto the surface of the wound and was then covered by an absorbent layer to contain the honey. Dressings were changed every alternate day for a week till there was complete cessation of pus discharge. Henceforth, the interval between dressings was increased to 1 week subsequently and was continued for 4 weeks. Assessment was done on the basis of discharge and depth of the wound before the procedure and weekly for 4 weeks.

          Results:

          The average depth of wound as seen at 15 sites after a week was 5.72 mm, and decrease in the average depth of wound seen at the end of the 4 th week was 0.88 mm with complete wound epithelization. This was found to be statistically significant ( P = 0.0001). No cases were reported with allergy, pain, infection, inflammation, and swelling on 1 st, 2 nd, 3 rd, and 4 thweek.

          Conclusion:

          Hence, the use of manuka honey as a wound dressing material in our study has proved to promote the growth of tissues for wound repair, suppress inflammation, and bring about rapid autolytic debridement.

          Related collections

          Most cited references19

          • Record: found
          • Abstract: found
          • Article: not found

          The unusual antibacterial activity of medical-grade Leptospermum honey: antibacterial spectrum, resistance and transcriptome analysis.

          There is an urgent need for new, effective agents in topical wound care, and selected honeys show potential in this regard. Using a medical-grade honey, eight species of problematic wound pathogens, including those with high levels of innate or acquired antibiotic resistance, were killed by 4.0-14.8% honey, which is a concentration that can be maintained in the wound environment. Resistance to honey could not be induced under conditions that rapidly induced resistance to antibiotics. Escherichia coli macroarrays were used to determine the response of bacterial cells to a sub-lethal dose of honey. The pattern of gene expression differed to that reported for other antimicrobial agents, indicating that honey acts in a unique and multifactorial way; 78 (2%) genes were upregulated and 46 (1%) genes were downregulated more than two-fold upon exposure to the medical-grade honey. Most of the upregulated genes clustered into distinct functional regulatory groups, with many involved in stress responses, and the majority of downregulated genes encoded for products involved in protein synthesis. Taken together, these data indicate that honey is an effective topical antimicrobial agent that could help reduce some of the current pressures that are promoting antibiotic resistance.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Manuka honey inhibits the development of Streptococcus pyogenes biofilms and causes reduced expression of two fibronectin binding proteins.

            Streptococcus pyogenes (group A Streptococcus; GAS) is always of clinical significance in wounds where it can initiate infection, destroy skin grafts and persist as a biofilm. Manuka honey has broad spectrum antimicrobial activity and its use in the clinical setting is beginning to gain acceptance with the continuing emergence of antibiotic resistance and the inadequacy of established systemic therapies; novel inhibitors may affect clinical practice. In this study, the effect of manuka honey on S. pyogenes (M28) was investigated in vitro with planktonic and biofilm cultures using MIC, MBC, microscopy and aggregation efficiency. Bactericidal effects were found in both planktonic cultures and biofilms, although higher concentrations of manuka honey were needed to inhibit biofilms. Abrogation of adherence and intercellular aggregation was observed. Manuka honey permeated 24 h established biofilms of S. pyogenes, resulting in significant cell death and dissociation of cells from the biofilm. Sublethal concentrations of manuka honey effectively prevented the binding of S. pyogenes to the human tissue protein fibronectin, but did not inhibit binding to fibrinogen. The observed inhibition of fibronectin binding was confirmed by a reduction in the expression of genes encoding two major fibronectin-binding streptococcal surface proteins, Sof and SfbI. These findings indicate that manuka honey has potential in the topical treatment of wounds containing S. pyogenes.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Effectiveness of honey on Staphylococcus aureus and Pseudomonas aeruginosa biofilms.

              Biofilms formed by Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) have been shown to be an important factor in the pathophysiology of chronic rhinosinusitis (CRS). As well, honey has been used as an effective topical antimicrobial agent for years. Our objective is to determine the in vitro effect of honey against biofilms produced by PA and SA. In vitro testing of honey against bacterial biofilms. We used a previously established biofilm model to assess antibacterial activity of honey against 11 methicillin-susceptible SA (MSSA), 11 methicillin-resistant SA (MRSA), and 11 PA isolates. Honeys were tested against both planktonic and biofilm-grown bacteria. Honey was effective in killing 100 percent of the isolates in the planktonic form. The bactericidal rates for the Sidr and Manuka honeys against MSSA, MRSA, and PA biofilms were 63-82 percent, 73-63 percent, and 91-91 percent, respectively. These rates were significantly higher (P<0.001) than those seen with single antibiotics commonly used against SA. Honey, which is a natural, nontoxic, and inexpensive product, is effective in killing SA and PA bacterial biofilms. This intriguing observation may have important clinical implications and could lead to a new approach for treating refractory CRS.
                Bookmark

                Author and article information

                Journal
                Natl J Maxillofac Surg
                Natl J Maxillofac Surg
                NJMS
                National Journal of Maxillofacial Surgery
                Wolters Kluwer - Medknow (India )
                0975-5950
                2229-3418
                May-Aug 2021
                15 July 2021
                : 12
                : 2
                : 233-237
                Affiliations
                [1]Department of Dentistry, Zydus Medical College and Hospital, Dahod, Gujarat, India
                [1 ]Department of Surgery, Zydus Medical College and Hospital, Dahod, Gujarat, India
                Author notes
                Address for correspondence: Dr. Nupur Kapoor, Department of Dentistry, Zydus Medical College and Hospital, Behind Bhagini Samaj, Dahod - 389 151, Gujarat, India. E-mail: nupur.sagacious@ 123456gmail.com
                Article
                NJMS-12-233
                10.4103/njms.NJMS_154_20
                8386265
                34483582
                98c50b02-f5d9-468d-9c12-ba2f551f1d41
                Copyright: © 2021 National Journal of Maxillofacial Surgery

                This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.

                History
                : 19 July 2020
                : 26 December 2020
                : 12 March 2021
                Categories
                Original Article

                Surgery
                debriding wounds,extraoral wounds,manuka honey
                Surgery
                debriding wounds, extraoral wounds, manuka honey

                Comments

                Comment on this article