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Derivation of human embryonic stem cells in defined conditions
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Abstract
We have previously reported that high concentrations of basic fibroblast growth factor
(bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those
conditions included poorly defined serum and matrix components. Here we report feeder-independent
human ES cell culture that includes protein components solely derived from recombinant
sources or purified from human material. We describe the derivation of two new human
ES cell lines in these defined culture conditions.