Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF).

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Abstract

Fibrin clot membranes prepared from advanced platelet-rich fibrin (A-PRF) or concentrated growth factors (CGF), despite their relatively rapid biodegradability, have been used as bioactive barrier membranes for alveolar bone tissue regeneration. As the membranes degrade, it is thought that the growth factors are gradually released. However, the mechanical and degradable properties of these membranes have not well been characterized. The purpose of this study was to mechanically and chemically characterize these membranes.

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Comparative release of growth factors from PRP, PRF, and advanced-PRF.

Eizaburo Kobayashi, Laura Flückiger, Masako Fujioka-Kobayashi … (2016)

The use of platelet concentrates has gained increasing awareness in recent years for regenerative procedures in modern dentistry. The aim of the present study was to compare growth factor release over time from platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and a modernized protocol for PRF, advanced-PRF (A-PRF).

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Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift.

Joseph Choukroun, Antoine Diss, Alain Simonpieri … (2006)

Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. The use of platelet gel to improve bone regeneration is a recent technique in implantology. However, the biologic properties and real effects of such products remain controversial. In this article, we therefore attempt to evaluate the potential of PRF in combination with freeze-dried bone allograft (FDBA) (Phoenix; TBF, France) to enhance bone regeneration in sinus floor elevation. Nine sinus floor augmentations were performed. In 6 sites, PRF was added to FDBA particles (test group), and in 3 sites FDBA without PRF was used (control group). Four months later for the test group and 8 months later for the control group, bone specimens were harvested from the augmented region during the implant insertion procedure. These specimens were treated for histologic analysis. Histologic evaluations reveal the presence of residual bone surrounded by newly formed bone and connective tissue. After 4 months of healing time, histologic maturation of the test group appears to be identical to that of the control group after a period of 8 months. Moreover, the quantities of newly formed bone were equivalent between the 2 protocols. Sinus floor augmentation with FDBA and PRF leads to a reduction of healing time prior to implant placement. From a histologic point of view, this healing time could be reduced to 4 months, but large-scale studies are still necessary to validate these first results.

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Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF)

Hideo Masuki, Toshimitsu Okudera, Taisuke Watanebe … (2016)

Background The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure of platelet-concentrated biomaterials, such as platelet-rich plasma (PRP), and facilitated their clinical application. PRF’s clinical effectiveness has often been demonstrated in pre-clinical and clinical studies; however, it is still controversial whether growth factors are significantly concentrated in PRF preparations to facilitate wound healing and tissue regeneration. To address this matter, we performed a comparative study of growth factor contents in PRP and its derivatives, such as advanced PRF (A-PRF) and concentrated growth factors (CGF). Methods PRP and its derivatives were prepared from the same peripheral blood samples collected from healthy donors. A-PRF and CGF preparations were homogenized and centrifuged to produce extracts. Platelet and white blood cell counts in A-PRF and CGF preparations were determined by subtracting those counts in red blood cell fractions, supernatant acellular serum fractions, and A-PRF/CGF exudate fractions from those counts of whole blood samples. Concentrations of growth factors (TGF-β1, PDGF-BB, VEGF) and pro-inflammatory cytokines (IL-1β, IL-6) were determined using ELISA kits. Results Compared to PRP preparations, both A-PRF and CGF extracts contained compatible or higher levels of platelets and platelet-derived growth factors. In a cell proliferation assay, both A-PRF and CGF extracts significantly stimulated the proliferation of human periosteal cells without significant reduction at higher doses. Conclusions These data clearly demonstrate that both A-PRF and CGF preparations contain significant amounts of growth factors capable of stimulating periosteal cell proliferation, suggesting that A-PRF and CGF preparations function not only as a scaffolding material but also as a reservoir to deliver certain growth factors at the site of application.

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Author and article information

Journal

Journal ID (iso-abbrev): Int J Implant Dent

Title: International journal of implant dentistry

Publisher: Springer Nature

ISSN (Print): 2198-4034

Publication date (Electronic): Dec 2017

Volume: 3

Issue: 1

Affiliations

[1 ] Tokyo Plastic Dental Society, Kita-ku, Tokyo, Japan.

[2 ] Division of Dental Implantology, Niigata University Medical and Dental Hospital, Niigata, Japan.

[3 ] Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan.

[4 ] Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata, Japan.

[5 ] Department of Materials Science and Technology, Niigata University, Niigata, Japan.

[6 ] Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan. kawase@dent.niigata-u.ac.jp.

Article

Publisher Item ID: 10.1186/s40729-017-0081-7

DOI: 10.1186/s40729-017-0081-7

PMC ID: 5413460

PubMed ID: 28466249

SO-VID: 57c1c15e-f6ab-43ca-a494-98dae62a64c9

History

Keywords: Concentrated growth factors,Degradability,Fibrin fiber,Platelet-poor plasma,Platelet-rich fibrin,Young’s modulus

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Keywords: Concentrated growth factors, Degradability, Fibrin fiber, Platelet-poor plasma, Platelet-rich fibrin, Young’s modulus

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Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF).

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High Entropy Alloys and Complex Concentrated Alloys

Most cited references 16

Comparative release of growth factors from PRP, PRF, and advanced-PRF.

Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift.

Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF)

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