Age influences the olfactory profiles of the migratory oriental armyworm mythimna separate at the molecular level

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

The oriental armyworm Mythimna separata (Walk) is a serious migratory pest; however, studies on its olfactory response and its underlying molecular mechanism are limited. To gain insights to the olfactory mechanism of migration, olfactory genes were identified using antennal transcriptome analysis. The olfactory response and the expression of olfactory genes for 1-day and 5-day-old moths were respectively investigated by EAG and RT-qPCR analyses.

Results

Putative 126 olfactory genes were identified in M. separata, which included 43 ORs, 13 GRs, 16 IRs, 37 OBPs, 14 CSPs, and 3 SNMPs. RPKM values of IR75d and 10 ORs were larger than co-receptors IR25a and ORco, and the RPKM value of PR2 was larger than that of other ORs. Expression of GR1 (sweet receptor) was higher than that of other GRs. Several sex pheromones activated evident EAG responses where the responses of 5-day-old male moths to the sex pheromones were significantly greater than those of female and 1-day old male moths. In accordance with the EAG response, 11 pheromone genes, including 6 PRs and 5 PBPs were identified in M. separate, and the expression levels of 7 pheromone genes in 5-day-old moths were significantly higher than those of females and 1-day-old moths. PR2 and PBP2 might be used in identifying Z11-16: Ald, which is the main sex pheromone component of M. separata. EAG responses to 16 plant volatiles and the expression levels of 43 olfactory genes in 1-day-old moths were significantly greater than that observed in the 5-day-old moths. Heptanal, Z6-nonenal, and benzaldehyde might be very important floral volatiles for host searching and recognized by several olfactory genes with high expression. Some plant volatiles might be important to male moths because the EAG response to 16 plant volatiles and the expression of 43 olfactory genes were significantly larger in males than in females.

Conclusions

The findings of the present study show the effect of adult age on olfactory responses and expression profile of olfactory genes in the migratory pest M. separate.

Electronic supplementary material

The online version of this article (doi:10.1186/s12864-016-3427-2) contains supplementary material, which is available to authorized users.

Related collections

Most cited references 74

Record: found
Abstract: found
Article: not found

Gene Ontology: tool for the unification of biology

Michael Ashburner, Catherine A. Ball, Judith Blake … (2002)

Genomic sequencing has made it clear that a large fraction of the genes specifying the core biological functions are shared by all eukaryotes. Knowledge of the biological role of such shared proteins in one organism can often be transferred to other organisms. The goal of the Gene Ontology Consortium is to produce a dynamic, controlled vocabulary that can be applied to all eukaryotes even as knowledge of gene and protein roles in cells is accumulating and changing. To this end, three independent ontologies accessible on the World-Wide Web (http://www.geneontology.org) are being constructed: biological process, molecular function and cellular component.

0 comments Cited 15604 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: found

Is Open Access

eggNOG v4.0: nested orthology inference across 3686 organisms

Sean Powell, Kristoffer Forslund, Damian Szklarczyk … (2013)

With the increasing availability of various ‘omics data, high-quality orthology assignment is crucial for evolutionary and functional genomics studies. We here present the fourth version of the eggNOG database (available at http://eggnog.embl.de) that derives nonsupervised orthologous groups (NOGs) from complete genomes, and then applies a comprehensive characterization and analysis pipeline to the resulting gene families. Compared with the previous version, we have more than tripled the underlying species set to cover 3686 organisms, keeping track with genome project completions while prioritizing the inclusion of high-quality genomes to minimize error propagation from incomplete proteome sets. Major technological advances include (i) a robust and scalable procedure for the identification and inclusion of high-quality genomes, (ii) provision of orthologous groups for 107 different taxonomic levels compared with 41 in eggNOGv3, (iii) identification and annotation of particularly closely related orthologous groups, facilitating analysis of related gene families, (iv) improvements of the clustering and functional annotation approach, (v) adoption of a revised tree building procedure based on the multiple alignments generated during the process and (vi) implementation of quality control procedures throughout the entire pipeline. As in previous versions, eggNOGv4 provides multiple sequence alignments and maximum-likelihood trees, as well as broad functional annotation. Users can access the complete database of orthologous groups via a web interface, as well as through bulk download.

0 comments Cited 281 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Odourant reception in the malaria mosquito Anopheles gambiae

Allison Carey, Guirong Wang, Chih-Ying Su … (2010)

Summary The mosquito Anopheles gambiae is the major vector of malaria in sub-Saharan Africa. It locates its human hosts primarily through olfaction, but little is known about the molecular basis of this process. Here we functionally characterize the Anopheles gambiae Odourant Receptor (AgOr) repertoire. We identify receptors that respond strongly to components of human odour and that may act in the process of human recognition. Some of these receptors are narrowly tuned, and some salient odourants elicit strong responses from only one or a few receptors, suggesting a central role for specific transmission channels in human host-seeking behavior. This analysis of the Anopheles gambiae receptors permits a comparison with the corresponding Drosophila melanogaster odourant receptor repertoire. We find that odourants are differentially encoded by the two species in ways consistent with their ecological needs. Our analysis of the Anopheles gambiae repertoire identifies receptors that may be useful targets for controlling the transmission of malaria.

0 comments Cited 225 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Yue-qiu He: heyueqiu@nbcc.cn

Bo Feng: fb@wzmc.edu.cn

Qian-shuang Guo: qianshuang123@126.com

Yongjun Du: 0086 577 86699500 , dyj@wzmc.edu.cn

Journal

Journal ID (nlm-ta): BMC Genomics

Journal ID (iso-abbrev): BMC Genomics

Title: BMC Genomics

Publisher: BioMed Central (London )

ISSN (Electronic): 1471-2164

Publication date (Electronic): 5 January 2017

Publication date PMC-release: 5 January 2017

Publication date Collection: 2017

Volume: 18

Electronic Location Identifier: 32

Affiliations

[1 ]Institute of Health and Environmental Ecology, Wenzhou Medical University, University Town, Wenzhou, 325035 China

[2 ]Ningbo City College of Vocational Technology, Xuefu Road, Yinzhou High Educational Park, NingBo, 315100 ZheJiang China

Article

Publisher ID: 3427

DOI: 10.1186/s12864-016-3427-2

PMC ID: 5217624

PubMed ID: 28056777

SO-VID: 538662f0-5659-47b6-8312-bc583954db25

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 28 July 2016

Date accepted : 15 December 2016

Funding

Funded by: Special Fund for Agro-scientific Research in the Public Interest in China

Award ID: 201203036

Award Recipient : Yongjun Du

Custom metadata

ScienceOpen disciplines: Genetics

Keywords: mythimna separata,migration,olfaction,pre-mating status,co-receptor,sex pheromone,host searching

Data availability:

ScienceOpen disciplines: Genetics

Keywords: mythimna separata, migration, olfaction, pre-mating status, co-receptor, sex pheromone, host searching

Age influences the olfactory profiles of the migratory oriental armyworm mythimna separate at the molecular level

Read this article at

Abstract

Background

Results

Conclusions

Electronic supplementary material

Related collections

G3: Genes|Genomes|Genetics

Most cited references 74

Gene Ontology: tool for the unification of biology

eggNOG v4.0: nested orthology inference across 3686 organisms

Odourant reception in the malaria mosquito Anopheles gambiae

Author and article information

Contributors

Journal

Affiliations

Article

History

Funding

Categories

Custom metadata

Comments

Comment on this article

Similar content 218

Cited by 12

Most referenced authors 1,912