Arbuscular Mycorrhizal Symbiosis Enhances Photosynthesis in the Medicinal Herb Salvia fruticosa by Improving Photosystem II Photochemistry

Abstract

We investigated the influence of Salvia fruticosa colonization by the arbuscular mycorrhizal fungi (AMF) Rhizophagus irregularis on photosynthetic function by using chlorophyll fluorescence imaging analysis to evaluate the light energy use in photosystem II (PSII) of inoculated and non-inoculated plants. We observed that inoculated plants used significantly higher absorbed energy in photochemistry (Φ _PSII ) than non-inoculated and exhibited significant lower excess excitation energy (EXC). However, the increased Φ _PSII in inoculated plants did not result in a reduced non-regulated energy loss in PSII (Φ _NO ), suggesting the same singlet oxygen ( ¹O ₂) formation between inoculated and non-inoculated plants. The increased Φ _PSII in inoculated plants was due to an increased efficiency of open PSII centers to utilize the absorbed light (F v’/F m’) due to a decreased non-photochemical quenching (NPQ) since there was no difference in the fraction of open reaction centers (q _p ). The decreased NPQ in inoculated plants resulted in an increased electron-transport rate (ETR) compared to non-inoculated. Yet, inoculated plants exhibited a higher efficiency of the water-splitting complex on the donor side of PSII as revealed by the increased F v/F o ratio. A spatial heterogeneity between the leaf tip and the leaf base for the parameters Φ _PSII and Φ _NPQ was observed in both inoculated and non-inoculated plants, reflecting different developmental zones. Overall, our findings suggest that the increased ETR of inoculated S. fruticosa contributes to increased photosynthetic performance, providing growth advantages to inoculated plants by increasing their aboveground biomass, mainly by increasing leaf biomass.