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Abstract
Karyotypic analysis of a metastatic malignant mixed tumor of the salivary gland revealed
the presence of double minute chromosomes (dmin), indicative of gene amplification.
Comparative genomic hybridization analysis of DNA extracted from the primary and a
renal metastasis indicated overt amplification of DNA sequences derived from 8q23-24
and 12q13-15 regions. Subsequent Southern blot analysis of tumor DNA from the metastasis
with the use of probes previously mapped to those regions indicated amplification
of MYC at 8q23-24 and CDK4 and MDM2 at 12q13-15. Fluorescence in situ hybridization
of differentially labeled MYC and MDM2 genes hybridized to tumor metaphase chromosomes
revealed an independent nonsyntenic amplification of MYC and MDM2 on dmin in this
tumor.