There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
This article has been retracted by Hindawi following an investigation undertaken by
the publisher [1]. This investigation has uncovered evidence of one or more of the
following indicators of systematic manipulation of the publication process:
Discrepancies in scope
Discrepancies in the description of the research reported
Discrepancies between the availability of data and the research described
Inappropriate citations
Incoherent, meaningless and/or irrelevant content included in the article
Peer-review manipulation
The presence of these indicators undermines our confidence in the integrity of the
article's content and we cannot, therefore, vouch for its reliability. Please note
that this notice is intended solely to alert readers that the content of this article
is unreliable. We have not investigated whether authors were aware of or involved
in the systematic manipulation of the publication process.
Wiley and Hindawi regrets that the usual quality checks did not identify these issues
before publication and have since put additional measures in place to safeguard research
integrity.
We wish to credit our own Research Integrity and Research Publishing teams and anonymous
and named external researchers and research integrity experts for contributing to
this investigation.
The corresponding author, as the representative of all authors, has been given the
opportunity to register their agreement or disagreement to this retraction. We have
kept a record of any response received.
Objective The ubiquitin-like with PHD and ring finger domains 1 (UHRF1) is a protein coding gene which is associated with colorectal cancer and other diseases. Therefore, the present study was aimed at investigating the effect and mechanism of UHRF1 protein on invasion and metastasis in human renal carcinoma cells. Methods After UHRF1 was interfered with or overexpressed in renal carcinoma cell lines A498 and 769-P, the relative mRNA and protein level of UHRF1 was detected by RT-qPCR and immunofluorescence. The colony formation assay and MTT were performed to observe the proliferation and cell viability in each group. In addition, the invasion and metastasis of the cells in each group were detected by Transwell and wound healing assay. Finally, Western blot was utilized to measure protein expression of MMP-2 and MMP-9 and the level of protein in the Wnt/ β -catenin signaling pathway. Results The cell ability, proliferation, invasion, and metastasis in A498 and 769-P cells were inhibited after interfering with UHRF1. In addition, the expression of MMP-2, MMP-9, c-myc, and β -catenin was significantly decreased, while the expression of GSK-3 β was significantly increased. However, contrasting results were demonstrated when UHRF1 was overexpressed. Conclusions Interference with the expression of UHRF1 was able to inhibit the invasion and metastasis of human renal carcinoma cell lines A498 and 769-P, which may be related to mediating the Wnt/ β -catenin signaling pathway and regulating the expression of MMP-2 and MMP-9.
This is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium,
provided the original work is properly cited.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.